The VH gene sequences of anti-DNA antibodies in two different strains of lupus-prone mice are highly related

European Journal of Immunology
D EilatJ F Bach

Abstract

The heavy and light chain V region sequences of an IgG anti-DNA autoantibody (PME77), derived from a lupus-prone (NZB x NZW)F1 mouse have been determined by mRNA sequencing. The V kappa gene segment belongs to the V kappa 1A gene sub-group and is found in several (NZB x NZW)F1 and MRL lpr/lpr anti-DNA antibodies, as well as in other antibodies of unrelated specificities. The VH gene segment appears to represent a unique gene or a subfamily of the large J558 VH gene family of the mouse, and is highly related to a germ-line sequence of a major anti-DNA idiotype (H130, IgM) of MRL mice. This anti-DNA-related VH segment has not been found, so far, to be expressed in antibodies with specificities for external or synthetic antigens; therefore, expression of such specificities may be regulated by powerful mechanisms of self tolerance in the healthy animal. In addition, both the heavy and light chain of the PME77 IgG antibody were found to contain somatic point mutations with a high ratio of replacement to silent mutations in complementarity determining regions. This IgM to IgG sequence relationship suggests an affinity maturation process, which is driven by the autoantigen.

References

May 1, 1975·CRC Critical Reviews in Biochemistry·B D Stollar
Aug 1, 1978·Clinical Immunology and Immunopathology·R D Sontheimer, J N Gilliam
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·L JacobD Louvard
Jul 1, 1985·The Journal of Experimental Medicine·R KoflerA N Theofilopoulos
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S C RileyR T Ogata
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S M Behar, M D Scharff
Oct 1, 1988·The Journal of Experimental Medicine·S E FergusonB A Osborne
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·L JacobD Louvard
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M J ShlomchikM G Weigert
Jun 1, 1987·The Journal of Experimental Medicine·D M Klinman, A D Steinberg
Aug 2, 1987·Nature·M J ShlomchikM G Weigert
Jan 1, 1985·Advances in Immunology·A N Theofilopoulos, F J Dixon
Apr 1, 1985·European Journal of Immunology·D EilatA Zlotnick
Mar 1, 1968·The Journal of Experimental Medicine·P H Lambert, F J Dixon
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·F TronJ F Bach
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·L JacobD Louvard
Jan 1, 1982·The Journal of Experimental Medicine·Y Kurosawa, S Tonegawa
Jan 1, 1981·Immunological Reviews·A D SteinbergE S Ravecheé
Apr 1, 1984·Immunology Today·R Dildrop

❮ Previous
Next ❯

Citations

Jan 1, 1994·Immunologic Research·T Kieber-EmmonsM P Madaio
Apr 1, 1989·Current Opinion in Immunology·B D Stollar
Sep 11, 2002·Molecular Immunology·Leian ChenChandra Mohan
Jan 7, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nuala J MeyerJoe G N Garcia
May 1, 2002·Current Opinion in Nephrology and Hypertension·Reshma Kewalramani, Ajay K Singh
Aug 1, 1992·Immunological Reviews·A K StewartR S Schwartz
Jun 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ute WellmannThomas H Winkler
Feb 1, 1991·Scandinavian Journal of Immunology·M E Alarcón-RiquelmeC Fernández
Nov 7, 1998·Seminars in Arthritis and Rheumatism·A RahmanD A Isenberg
Feb 1, 1993·European Journal of Immunology·E RazD Eilat
Feb 1, 1995·Clinical and Experimental Immunology·M E Alarcón-Riquelme, C Fernández
Sep 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zhiyan LiangChandra Mohan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.