The Vibrio cholerae FlgM homologue is an anti-sigma28 factor that is secreted through the sheathed polar flagellum

Journal of Bacteriology
N E CorreaK E Klose

Abstract

Vibrio cholerae has a single polar sheathed flagellum that propels the cells of this bacterium. Flagellar synthesis, motility, and chemotaxis have all been linked to virulence in this human pathogen. V. cholerae expresses flagellar genes in a hierarchy consisting of sigma54- and sigma28-dependent transcription. In other bacteria, sigma28 transcriptional activity is controlled by an anti-sigma28 factor, FlgM. We demonstrate that the V. cholerae FlgM homologue (i) physically interacts with sigma28, (ii) has a repressive effect on some V. cholerae sigma28-dependent flagellar promoters, and (iii) is secreted through the polar sheathed flagellum, consistent with anti-sigma28 activity. Interestingly, FlgM does not have a uniform repressive effect on all sigma28-dependent promoters, as determined by measurement of sigma28-dependent transcription in cells either lacking FlgM (DeltaflgM) or incapable of secretion (DeltafliF). Further analysis of a DeltafliF strain revealed that this flagellar assembly block causes a decrease in class III (FlrC- and sigma54-dependent) and class IV (sigma28-dependent), but not class II (FlrA- and sigma54-dependent), flagellar transcription. V. cholerae flgM and fliA (encodes sigma28) mutants were only mod...Continue Reading

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Citations

Jun 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·D Ewen CameronJohn J Mekalanos
Sep 22, 2009·Journal of Bacteriology·Manuel MoisiStefan Schild
Sep 1, 2009·Journal of Bacteriology·Khalid Ali SyedKarl E Klose
Apr 20, 2005·Journal of Bacteriology·Nidia E Correa, Karl E Klose
Jul 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Zhi LiuJun Zhu
Jan 29, 2013·Biochimica Et Biophysica Acta·Wai Kit MaMatthew J Gage
Mar 28, 2018·Journal of Bacteriology·Mylea A EchazarretaKarl E Klose
Mar 5, 2010·Molecular Microbiology·Marc M S M WöstenJos P M van Putten
Jul 9, 2011·Molecular Microbiology·Yi-Huang HsuehDaniel B Kearns
Jun 27, 2020·PLoS Genetics·Avatar JoshiFitnat H Yildiz
May 24, 2019·Frontiers in Cellular and Infection Microbiology·Mylea A Echazarreta, Karl E Klose
Aug 21, 2020·Applied Microbiology and Biotechnology·Fazlurrahman KhanYoung-Mog Kim
Jul 12, 2019·Frontiers in Cellular and Infection Microbiology·Nina PennetzdorferStefan Schild
Jun 24, 2021·Proceedings of the National Academy of Sciences of the United States of America·Kevin ManeraTao G Dong
Dec 4, 2021·Environmental Microbiology·Antonio Leal-MoralesFernando Govantes

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