The Vibrio cholerae RND efflux systems impact virulence factor production and adaptive responses via periplasmic sensor proteins

PLoS Pathogens
X Renee BinaJames E Bina

Abstract

Resistance-nodulation-division (RND) efflux systems are ubiquitous transporters in Gram-negative bacteria that are essential for antibiotic resistance. The RND efflux systems also contribute to diverse phenotypes independent of antimicrobial resistance, but the mechanism by which they affect most of these phenotypes is unclear. This is the case in Vibrio cholerae where the RND systems function in antimicrobial resistance and virulence factor production. Herein, we investigated the linkage between RND efflux and V. cholerae virulence. RNA sequencing revealed that the loss of RND efflux affected the activation state of periplasmic sensing systems including the virulence regulator ToxR. Activation of ToxR in an RND null mutant resulted in ToxR-dependent transcription of the LysR-family regulator leuO. Increased leuO transcription resulted in the repression of the ToxR virulence regulon and attenuated virulence factor production. Consistent with this, leuO deletion restored virulence factor production in an RND-null mutant, but not its ability to colonize infant mice; suggesting that RND efflux was epistatic to virulence factor production for colonization. The periplasmic sensing domain of ToxR was required for the induction of leu...Continue Reading

References

Dec 1, 1987·Journal of Clinical Microbiology·M Iwanaga, T Kuyyakanond
Jan 1, 1995·Clinical Microbiology Reviews·J B KaperM M Levine
Nov 14, 1997·Molecular Microbiology·K Skorupski, R K Taylor
Jan 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·C C Häse, J J Mekalanos
Aug 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·D Provenzano, K E Klose
Jun 13, 2001·Infection and Immunity·J E Bina, J J Mekalanos
Apr 26, 2002·The Journal of Biological Chemistry·Sheng Jian Cai, Masayori Inouye
Jun 7, 2002·Nature·D Scott MerrellAndrew Camilli
Jul 11, 2002·Journal of Bacteriology·Anna D TischlerAndrew Camilli
Feb 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·James BinaJohn Mekalanos
Feb 28, 2003·The Journal of Biological Chemistry·Valérie C SimonetAnne H Delcour
Jun 29, 2006·Archives of Microbiology·James E BinaJohn J Mekalanos
Jul 18, 2006·Nature Reviews. Microbiology·Laura J V Piddock
Jul 31, 2007·Future Microbiology·Brandon M Childers, Karl E Klose
Sep 9, 2008·Journal of Bacteriology·Francisca A Cerda-MairaRonald K Taylor
Jun 23, 2009·Journal of Bacteriology·Leyla Slamti, Matthew K Waldor
Jun 30, 2009·Nature Chemical Biology·Bryson D BennettJoshua D Rabinowitz
Sep 17, 2009·Nature Reviews. Microbiology·Eric J NelsonAndrew Camilli
Dec 7, 2011·Infection and Immunity·Alexandra R MeyShelley M Payne
May 17, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jessica V HankinsM Stephen Trent
Feb 7, 2013·Frontiers in Microbiology·Carolina Alvarez-OrtegaJosé L Martínez
Sep 18, 2013·The Journal of Antimicrobial Chemotherapy·Cristian Ruiz, Stuart B Levy
Mar 20, 2014·Microbiology·Amit VikramJames E Bina
Mar 20, 2015·Clinical Microbiology Reviews·Xian-Zhi LiHiroshi Nikaido
Apr 1, 2015·Infection and Immunity·Nicole AcostaTracy L Raivio
Jun 11, 2016·Biochemical Society Transactions·Antony J PalmerStephen P Muench
Aug 24, 2016·Infection and Immunity·X Renee BinaJames E Bina

❮ Previous
Next ❯

Citations

Dec 21, 2019·Biotechnology Journal·Austin G RottinghausTae Seok Moon
Feb 20, 2020·Future Microbiology·Abigail L ColcloughJessica Ma Blair
May 1, 2020·Japanese Journal of Infectious Diseases·Taniya GolderRanjan Kumar Nandy
Jan 9, 2019·Infection and Immunity·Mondraya F HowardJames E Bina
Apr 15, 2020·Infection and Immunity·D E KunkleJ E Bina
Oct 27, 2020·Frontiers in Cellular and Infection Microbiology·Thandavarayan RamamurthyAmit Ghosh
Jul 27, 2021·Infection and Immunity·Yuding WengJames E Bina
Sep 21, 2021·Infection and Immunity·Thomas F BinaJames E Bina

❮ Previous
Next ❯

Datasets Mentioned

BETA
SRP109296

Methods Mentioned

BETA
RNAseq
PCR
RNA-Seq
enzyme-linked immunosorbent CT
Assay
electrophoresis
ELISA

Software Mentioned

RNAseq
CLC Genomics Workbench RNA - Seq Analysis
CLC Genomics Workbench

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Bacterial Transport Proteins

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Bacterial Transport Proteins (ASM)

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.