PMID: 9188594Jul 1, 1997Paper

The Vif and Gag proteins of human immunodeficiency virus type 1 colocalize in infected human T cells

Journal of Virology
J H SimonM H Malim

Abstract

The Vif protein of human immunodeficiency virus type 1 (HIV-1) and other lentiviruses is required for efficient replication in primary cells and certain immortalized cell lines in vitro and, in all likelihood, for the establishment of pathogenic infections in vivo. Current hypotheses concerning Vif's mechanism of action posit that it operates in virus-expressing cells during virion assembly, budding, or maturation such that released virions are modified in a manner that enables them to undergo productive infection in subsequent viral challenges. To gain further insight into the mechanism of action of lentivirus Vif proteins, we have performed a variety of in situ localization and biochemical fractionation studies using cells in which Vif is essential for efficient replication. Double-label immunofluorescence analyses of cells productively infected with HIV-1 or feline immunodeficiency virus revealed dramatic patterns of colocalization between Vif and the virally encoded Gag proteins. Subcellular fractionations of human T cells expressing HIV-1 Vif performed in the absence of any detergent demonstrated that greater than 90% of Vif is associated with cellular membranes. Additional purification using a continuous density gradient ...Continue Reading

References

Jan 1, 1992·Virus Genes·M S Oberste, M A Gonda
Sep 1, 1992·Virology·L Fan, K Peden
Oct 1, 1990·Journal of Virology·T R PhillipsJ H Elder
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·H G GöttlingerW A Haseltine
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R A OlmstedP R Johnson
May 1, 1994·AIDS Research and Human Retroviruses·J S GibbsR C Desrosiers
Nov 1, 1994·Virology·B L Shacklett, P A Luciw
Jul 1, 1994·Genes & Development·B E Meyer, M H Malim
Dec 1, 1994·Journal of Virology·T DorfmanH G Göttlinger
Jun 1, 1994·Virology·S HöglundD Gabuzda
Feb 1, 1994·Journal of Virology·J GoncalvesD H Gabuzda
Apr 2, 1996·Proceedings of the National Academy of Sciences of the United States of America·C P HillW I Sundquist
Dec 1, 1996·Journal of Virology·J GoncalvesD Gabuzda

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Citations

Aug 1, 1998·AIDS Research and Human Retroviruses·F Bibollet-RucheM Peeters
Feb 23, 2000·Journal of Virology·U ChatterjiJ H Elder
Mar 30, 2005·Journal of Virology·Balaji ManicassamyLijun Rong
Jul 8, 2005·Proceedings of the National Academy of Sciences of the United States of America·April J SchumacherReuben S Harris
Aug 19, 2007·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Shixing TangIndira K Hewlett
Oct 16, 2009·Journal of Virology·Melody M H LiMichael Emerman
Feb 7, 1998·AIDS Research and Human Retroviruses·L Al-HarthiS K Arya
Nov 9, 2000·Journal of Virology·A Ohagen, D Gabuzda
Dec 10, 1998·Nature Medicine·J H SimonM H Malim
Jul 25, 2000·Journal of Virology·R J MillerN Sarver
Mar 30, 2001·The Journal of Biological Chemistry·G HassaïneE Decroly
Feb 17, 2009·Protein Engineering, Design & Selection : PEDS·Tali H ReingewertzAssaf Friedler
Jun 18, 2004·The EMBO Journal·Chad M SwansonMichael H Malim
Oct 29, 1998·The Journal of Biological Chemistry·X Yang, D Gabuzda
Feb 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Keiko SakaiMichael J Lenardo
Mar 7, 2009·PLoS Pathogens·Hendrik HuthoffMichael H Malim

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