The villus gradient of brush border membrane calmodulin and the calcium-independent calmodulin-binding protein parallels that of calcium-accumulating ability

Endocrinology
D D Bikle, S Munson

Abstract

We have recently proposed that calmodulin (CaM) may mediate calcium transport across the intestinal brush border membrane. Since calcium transport across this membrane varies as a function of cellular location on the villus (the highest rates of transport occur across the brush border membrane from cells near the tip), we tested this hypothesis by determining whether CaM and its principal binding protein in the brush border membrane [a 102,000 mol wt (102K) protein] also showed this gradient of activity along the villus. Cells were sequentially eluted from the tip to the base of the villus, brush border membrane vesicles (BBMV) were prepared from the eluted cells, and CaM, CaM binding, and calcium-accumulating ability were determined for each preparation of BBMV. We observed that BBMV prepared from cells originating near the tip of the villus possessed the greatest calcium-accumulating activity, CaM content, and CaM binding by the 102K protein. All three measurements were reduced in parallel in BBMV prepared from cells originating from more basal regions of the villus. Calcium-accumulating ability correlated with CaM content (r = 0.876) and CaM binding to the 102K protein (r = 0.788); likewise, CaM correlated with CaM binding t...Continue Reading

Citations

Feb 1, 1989·Pflügers Archiv : European journal of physiology·P A LucasT Drüeke
Jun 24, 1987·Biochimica Et Biophysica Acta·C H van Os
Apr 26, 2007·Kidney International·J MarksUNKNOWN Epithelial Transport and Cell Biology Group
Aug 11, 2012·Endocrinology and Metabolism Clinics of North America·Ronald D Emkey, Gregory R Emkey
Jun 11, 2010·American Journal of Physiology. Renal Physiology·Joanne MarksRobert J Unwin
Apr 13, 1999·The American Journal of Physiology·J C Fleet, R J Wood
Dec 12, 2001·American Journal of Physiology. Renal Physiology·Emmanuel K O Siaw, Marian R Walters
Oct 18, 2019·Journal of the Endocrine Society·Scott MunsonDaniel D Bikle

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