The vimE gene downstream of vimA is independently expressed and is involved in modulating proteolytic activity in Porphyromonas gingivalis W83

Infection and Immunity
E VanterpoolH M Fletcher

Abstract

Regulation/activation of the Porphyromonas gingivalis gingipains is poorly understood. A unique 1.3-kb open reading frame downstream of the bcp-recA-vimA transcriptional unit was cloned, insertionally inactivated with the ermF-ermAM antibiotic resistance cassette, and used to create a defective mutant by allelic exchange. In contrast to the wild-type W83 strain, the growth rate of the mutant strain (designated FLL93) was reduced, and when plated on Brucella blood agar it was nonpigmented and nonhemolytic. Arginine- and lysine-specific gingipain activities were reduced by approximately 90 and 85%, respectively, relative to activities of the parent strain. These activities were unaffected by the culture's growth phase, in contrast to the vimA-defective mutant P. gingivalis FLL92, which has increased proteolytic activity in stationary phase. Expression of the rgpA, rgpB, and kgp gingipain genes was unaltered in P. gingivalis FLL93 compared to that of the wild-type strain. Further, in extracellular protein fractions a 64-kDa band was identified that was immunoreactive with the RgpB-specific proenzyme antibodies. Active-site labeling with dansyl-glutamyl-glycyl-arginyl chloromethyl ketone or immunoblot analysis showed no detectable ...Continue Reading

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Citations

Nov 9, 2012·Molecular Oral Microbiology·D OsbourneH M Fletcher
Feb 26, 2005·Infection and Immunity·Elaine VanterpoolHansel M Fletcher
Oct 14, 2008·Journal of Bacteriology·Leroy G HenryHansel M Fletcher
May 7, 2009·Future Microbiology·Rebecca E FitzpatrickRobert N Pike
Mar 24, 2012·Future Microbiology·Leroy G HenryHansel M Fletcher
Jun 21, 2007·Microbiology and Immunology·Keitarou Saiki, Kiyoshi Konishi
Jan 3, 2013·Molecular Oral Microbiology·A W AruniH M Fletcher
Nov 20, 2015·Microbiology·Rachelle M E McKenzieHansel M Fletcher
Apr 10, 2010·Microbiology·Devon O OsbourneHansel M Fletcher
Sep 27, 2018·Journal of Bacteriology·Arunima MishraHansel M Fletcher

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