The Viral Hemorrhagic Septicemia Virus (VHSV) Markers of Virulence in Rainbow Trout (Oncorhynchus mykiss )

Frontiers in Microbiology
Laury BaillonStéphane Biacchesi

Abstract

Viral hemorrhagic septicemia virus (VHSV) is a highly contagious virus leading to high mortality in a large panel of freshwater and marine fish species. VHSV isolates originating from marine fish show low pathogenicity in rainbow trout. The analysis of several nearly complete genome sequences from marine and freshwater isolates displaying varying levels of virulence in rainbow trout suggested that only a limited number of amino acid residues might be involved in regulating the level of virulence. Based on a recent analysis of 55 VHSV strains, which were entirely sequenced and phenotyped in vivo in rainbow trout, several amino acid changes putatively involved in virulence were identified. In the present study, these amino acid changes were introduced, alone or in combination, in a highly-virulent VHSV 23-75 genome backbone by reverse genetics. A total of 35 recombinant VHSV variants were recovered and characterized for virulence in trout by bath immersion. Results confirmed the important role of the NV protein (R116S) and highlighted a major contribution of the nucleoprotein N (K46G and A241E) in regulating virulence. Single amino acid changes in these two proteins drastically affect virus pathogenicity in rainbow trout. This is...Continue Reading

References

Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T R FuerstB Moss
Jan 1, 1997·The Journal of General Virology·G KurathH V Björklund
Jul 19, 2000·The Journal of General Virology·S BiacchesiM Brémont
Jul 24, 2002·Nucleic Acids Research·Kazutaka KatohTakashi Miyata
Mar 30, 2004·Journal of Virology·Maria-Isabel ThoulouzeMichel Brémont
Apr 24, 2004·The Journal of General Virology·Katja Einer-JensenNiels Lorenzen
Dec 9, 2004·Diseases of Aquatic Organisms·M SnowR S Raynard
Feb 5, 2010·Journal of Virology·Tatsunori MasataniMakoto Sugiyama
Apr 23, 2010·Veterinary Research·Peter J Walker, James R Winton
Feb 15, 2011·Veterinary Research·Stéphane Biacchesi
Mar 24, 2012·Current Opinion in Virology·Gael Kurath, James Winton
Dec 18, 2013·Diseases of Aquatic Organisms·Eveline J EmmeneggerGael Kurath
Jan 9, 2016·Veterinary Research·Takafumi ItoNiels J Olesen
Apr 12, 2016·Molecular BioSystems·Jillian N WhelanMichael N Teng
Sep 29, 2020·Frontiers in Microbiology·Valentina PanzarinNiels J Olesen

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BETA
PCR
transfection

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MAFFT
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