PMID: 2093391Jan 1, 1990Paper

The viscosity of neutrophils and their transit times through small pores

Biorheology
R M Hochmuth, D Needham

Abstract

Passive neutrophils from five different individuals are rapidly aspirated at constant suction pressure and at room temperature into a pipet with a diameter of 4 microns. The excess suction pressures (i.e., the pressures in excess of the small threshold pressure required to produce continuous flow into the pipet) are 5000, 10,000 and 20,000 dyn/cm2 (0.5, 1 and 2 kPa) and are comparable to those encountered in the microcirculation. The rate of entry into the pipet is modeled with a linearized version of a theory by Yeung and Evans for the newtonian flow of a neutrophil into a pipet or pore. From this theory and measurements of the cell size and its rate of entry into the pipet, we can calculate a value for the cytoplasmic viscosity. A linear (newtonian) fit of the theory to the experimental data gives a value for the viscosity of 1050 poise. A non-linear fit predicts a decrease in the "apparent viscosity" from about 1500 poise at zero excess pressure to 1000 poise at an excess aspiration pressure of 20,000 dyn/cm2. Our experiments and analysis also allow us to calculate a value for the transit time through short pores over a wide range of excess aspiration pressures and pore diameters. For example, for a pore diameter of 3 micron...Continue Reading

Citations

Aug 27, 2005·Journal of Applied Physiology·Belinda Yap, Roger D Kamm
Apr 12, 2008·Physical Biology·Comron NouriRichard Gordon
Dec 3, 2015·Scientific Reports·Dongping QiAmy C Rowat
Jan 21, 2018·Annals of Biomedical Engineering·Herbert H LipowskyJustin L Brown
May 10, 2013·PloS One·Frank A W CoumansLeon W M M Terstappen
Oct 20, 1991·Biotechnology and Bioengineering·D NeedhamR Tran-Son-Tay

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