PMID: 7528011Dec 1, 1994Paper

The vitronectin receptor (alpha v beta 3) is implicated, in cooperation with P-selectin and platelet-activating factor, in the adhesion of monocytes to activated endothelial cells

The Biochemical Journal
J F MurphyJ L McGregor

Abstract

In this study we have investigated the presence on endothelial cells of potential glycoprotein receptors, other than P-selectin, which are involved in the adhesion of monocytes at the early stages of activation. We report that the majority of cells binding to thrombin-activated endothelial cells from a peripheral blood mononuclear cell (PBMC) preparation are monocytes. The adhesion of PBMC to thrombin-activated, but not resting, endothelial cells was inhibited (66%) by a monoclonal antibody (mAb) directed against alpha v beta 3. Elutriated monocytes or a monocytic cell line (U937) were also inhibited by this antibody, its F(ab)'2 fragments and three other anti-(alpha v beta 3) mAbs. alpha v beta 3 isolated from endothelial-cell lysates significantly inhibited the adhesion of monocytes and U937 cells to endothelial cells. A peptide motif (RGDF) known to interact with alpha v beta 3 inhibited U937 cell adhesion to activated endothelial cells by 53%. Finally, an anti-(P-selectin) mAb (LYP20) or a platelet-activating factor (PAF)-receptor antagonist (WEB 2086) inhibited monocyte adhesion to activated endothelial cells. This study shows for the first time that alpha v beta 3 is implicated, in addition to P-selectin and PAF, in the a...Continue Reading

Citations

Apr 3, 2003·British Journal of Haematology·Joseph F MurphyDesmond J Fitzgerald
Aug 30, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·G A Stouffer, S S Smyth
Jul 26, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Shigeru IchiokaTakashi Nakatsuka
Oct 8, 2009·Circulation. Cardiovascular Imaging·Matthias NahrendorfAlbert J Sinusas
Mar 10, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anna BertramKlaus Ley
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Aug 1, 2006·Pathophysiology of Haemostasis and Thrombosis·Patrizia FerroniGiovanni Davì
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Aug 18, 2006·Basic Research in Cardiology·Sanjiv Kaul
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