The voltage-dependent activity of Escherichia coli porins in different planar bilayer reconstitutions.

European Journal of Biochemistry
J H Lakey, F Pattus

Abstract

Because of conflicting results from differing techniques, the degree of voltage sensitivity of Escherichia coli porins in planar bilayers is still a matter of debate. In order to provide the first comparative study, OmpF porin was purified in three ways; firstly as native outer membrane vesicles, secondly as salt-extracted porin trimers in sodium dodecyl sulphate and thirdly as solubilised trimers extracted with octyl-polyoxyethylene (Octyl-POE). These methods represent the major approaches to porin isolation and purification. All three were reconstituted into Schindler-type bilayers. Detergent-solubilised OmpF was also reconstituted into Montal-Mueller- and Mueller-Rudin-type bilayers. In all cases voltage-dependent closing of OmpF was observed. Octyl-POE-extracted PhoE porin was similarly investigated in all three types of planar bilayer. Two membrane-formation techniques appeared genuinely to alter the voltage sensitivity of the porins they contained. Firstly, porins in membranes formed by the Montal-Mueller technique sometimes showed an increase in voltage sensitivity during the first 30 min after bilayer formation. Secondly, membranes formed by the Mueller-Rudin technique on thick polyethylene septa showed both poor solven...Continue Reading

References

Feb 2, 1978·Biochimica Et Biophysica Acta·F PattusR Verger
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H Schindler, J P Rosenbusch
Jul 3, 1975·Biochimica Et Biophysica Acta·R BenzM Montal
Jan 1, 1986·Methods in Enzymology·R M Garavito, J P Rosenbusch
Nov 6, 1986·Biochimica Et Biophysica Acta·G Z XuH T Tien
Jan 19, 1987·FEBS Letters·J H Lakey
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B MartinacC Kung
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A MauroP Labarca
Apr 15, 1988·Biochemical and Biophysical Research Communications·L HymelS Fleischer
Jan 1, 1987·The Journal of Membrane Biology·R BenzG H Vos-Scheperkeuter
Dec 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·M Montal, P Mueller
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·H Schindler, J P Rosenbusch
Nov 1, 1983·Journal of Bacteriology·J SutcliffeJ Foulds
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J D YoungZ A Cohn
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·H Schindler, U Quast

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Citations

Jul 16, 2009·European Biophysics Journal : EBJ·M MohammadzadehF Arazm
May 29, 2009·Molecular & Cellular Proteomics : MCP·Yi ZhuSiu Kwan Sze
Feb 28, 1998·The EMBO Journal·H Samartzidou, A H Delcour
Feb 15, 1993·European Journal of Biochemistry·M Brunen, H Engelhardt
Sep 24, 1999·Biological Chemistry·A WieseS Müller-Loennies
Jul 27, 2002·Protein Science : a Publication of the Protein Society·Samuel TerrettazJeremy H Lakey
Nov 1, 1990·Quarterly Reviews of Biophysics·B K Jap, P J Walian
Dec 3, 2014·Biochimica Et Biophysica Acta·Carolina L GoulartFabrice Homblé
Oct 1, 1995·Kidney International·W WelteK Diederichs
Jul 9, 2004·Biochimica Et Biophysica Acta·Arnaud BasléAnne H Delcour
Sep 15, 2010·The Biochemical Journal·Kornelius Zeth, Marcus Thein
Sep 29, 2011·Biochimica Et Biophysica Acta·Daniel FologeaGreg J Salamo
Jul 31, 2012·Structure·Ulrich ZachariaeAdam Lange
Jun 20, 2006·Biophysical Journal·Eva K SchmittClaudia Steinem
May 9, 2015·PLoS Computational Biology·Wanling SongYechun Xu
Mar 29, 2000·Journal of Molecular Biology·R IyerA H Delcour
Nov 3, 1992·Biochemistry·J C TodtE J McGroarty
Feb 19, 1998·Journal of Molecular Biology·G BainbridgeJ H Lakey
Feb 5, 1996·FEBS Letters·N D BishopS Spiro
Jun 15, 1997·FEMS Microbiology Letters·A H Delcour
Jun 6, 1998·Journal of Structural Biology·T Schirmer
Jan 1, 1993·Journal of Bacteriology·G ChevalierH Wróblewski
Jul 1, 1996·Journal of Bacteriology·A L Dela Vega, A H Delcour
Jul 1, 1996·Journal of Bacteriology·M SimónH Engelhardt
Jan 18, 1993·Biochimica Et Biophysica Acta·N SaintG Molle
Jan 19, 1994·Biochimica Et Biophysica Acta·D DahanJ W Coulton
Jul 31, 1992·Biochemical and Biophysical Research Communications·M Obara, T Nakae

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