The voltage sensor of excitation-contraction coupling in skeletal muscle. Ion dependence and selectivity.

The Journal of General Physiology
G PizarroE Ríos

Abstract

Manifestations of excitation-contraction (EC) coupling of skeletal muscle were studied in the presence of metal ions of the alkaline and alkaline-earth groups in the extracellular medium. Single cut fibers of frog skeletal muscle were voltage clamped in a double Vaseline gap apparatus, and intramembrane charge movement and myoplasmic Ca2+ transients were simultaneously measured. In metal-free extracellular media both charge movement of the charge 1 type and Ca transients were suppressed. Under metal-free conditions the nonlinear charge distribution was the same in depolarized (holding potential of 0 mV) and normally polarized fibers (holding potentials between -80 and -90 mV). The manifestations of EC coupling recovered when ions of groups Ia and IIa of the periodic table were included in the extracellular solution; the extent of recovery depended on the ion species. These results are consistent with the idea that the voltage sensor of EC coupling has a binding site for metal cations--the "priming" site--that is essential for function. A state model of the voltage sensor in which metal ligands bind preferentially to the priming site when the sensor is in noninactivated states accounts for the results. This theory was used to de...Continue Reading

Citations

Jan 8, 2010·European Biophysics Journal : EBJ·Ricky C K ChengBoris S Zhorov
Jul 20, 2011·Biophysical Journal·Roman Shirokov
Jan 13, 2010·Molecular and Biochemical Parasitology·Sreekanth PuttacharyRichard J Martin
Oct 13, 2017·The Journal of General Physiology·Juan Ferreira GregorioGustavo Brum
Aug 10, 2006·Journal of Muscle Research and Cell Motility·Werner MelzerDaniel Ursu
Jul 3, 2010·Journal of Muscle Research and Cell Motility·J Fernando Olivera, Gonzalo Pizarro
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