PMID: 8596697Feb 1, 1996Paper

The volume-activated chloride current in endothelial cells from bovine pulmonary artery is not modulated by phosphorylation

Pflügers Archiv : European journal of physiology
G SzücsB Nilius

Abstract

We employed the patch-clamp technique to investigate the effects of various phosphorylation pathways on activation and modulation of volume-activated Cl- currents (ICl,vol) in cultured endothelial cells from bovine pulmonary arteries (CPAE cells). Half-maximal activation of ICl,vol occurred at a hypotonicity of 27.5+/-1.2%. Run-down of the current upon repetitive activation was less than 15% within 60 min. Stimulation of protein kinase C (PKC) by phorbol-12-myristate-13-acetate (PMA) or by (-)-indolactam did not affect ICl,vol. Down regulation of PKC activity by a 24-h preincubation of the cells with 0.2 micromol/l PMA, or its inhibition by loading the cells with the specific inhibitory 19-31 pseudosubstrate peptide, did not influence ICl,vol. Trifluoperazine and tamoxifen fully blocked ICl,vol with concentrations required for half-maximal inhibition of 3.0 and 2.4 micromol/l respectively. This inhibitory effect is probably not mediated by the calmodulin-antagonistic action of these compounds, because it occurs at free intracellular [Ca2+] of 50 nmol/l, which are below the threshold for calmodulin activation. The tyrosine kinase inhibitor herbimycin A (1 micromol/l) and genistein (100 micromol/l) did not affect ICl,vol. Exposin...Continue Reading

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Citations

Jan 1, 1997·Progress in Biophysics and Molecular Biology·B NiliusG Droogmans
Oct 1, 1996·General Pharmacology·B NiliusG Droogmans
Mar 27, 2001·British Journal of Pharmacology·M L DoddsG J Kargacin
Jan 1, 1997·Annual Review of Physiology·B NiliusG Droogmans
Jul 26, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Kenneth B Walsh, Jining Zhang
Mar 28, 2002·Physiological Reviews·Thomas J JentschAnselm A Zdebik
Jul 1, 1997·The American Journal of Physiology·V G ManolopoulosB Nilius
Aug 1, 1997·Journal of Applied Physiology·M P Griffin
Jun 5, 1998·The American Journal of Physiology·F EmmaK Strange
Sep 26, 2000·American Journal of Physiology. Cell Physiology·Y M ShubaR Skryma
Oct 23, 2001·American Journal of Physiology. Heart and Circulatory Physiology·M J DavisE Wilson
Jun 13, 2001·American Journal of Physiology. Cell Physiology·A Y EstevezK Strange
Nov 29, 1996·The Journal of Biological Chemistry·B NiliusG Droogmans

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