The von Hippel-Lindau (VHL) tumor-suppressor gene is down-regulated by selenium deficiency in Caco-2 cells and rat colon mucosa

Biological Trace Element Research
Eric O UthusHuawei Zeng

Abstract

To test the hypothesis that selenium affects DNA methylation and hence gene regulation, we employed a methylation array (Panomics) in the human colonic epithelial Caco-2 cell model. The array profiles DNA methylation from promoter regions of 82 human genes. After conditioning cells to repeatedly reduced concentrations of fetal bovine serum, a serum-free culture was established. Se-methylselenocysteine (SeMSC) was added at 0 (deficient Se) or 250 (control Se) nM to cells maintained in DMEM. After 7 days, cells were collected and stored at -80 °C until analysis; experiments were replicated three times. Glutathione peroxidase activity was significantly decreased in cells grown in low SeMSC. Cells grown in 250 nM SeMSC had maximal GPx activity. Genomic DNA from cells grown in the low-SeMSC media and media containing 250 nM SeMSC was incubated with methylation-binding protein followed by isolation of methylated DNA. The methylated DNA was labeled with biotin and hybridized to the methylation array. Thus, genes with promoter methylation will produce a higher chemiluminescence signal than those genes with no promoter methylation. Of the genes profiled, the von Hippel-Lindau (VHL) gene was most different as indicated by quantification ...Continue Reading

References

Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·W L McKeehanR G Ham
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M FrommerC L Paul
Jul 16, 1983·Lancet·W C WillettC G Hames
Sep 1, 1984·American Journal of Epidemiology·J T SalonenP Puska
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·J G HermanW M Linehan
Oct 1, 1996·Genome Research·C A HeidP M Williams
May 1, 1997·The Journal of Nutrition·P G Reeves
Mar 24, 2000·Trends in Genetics : TIG·S B Baylin, J G Herman
Jul 27, 2000·Annals of the New York Academy of Sciences·J P Issa
Apr 2, 2002·The Journal of Nutrition·Huawei Zeng
Apr 3, 2003·Nature Reviews. Cancer·Peter W Laird
May 6, 2004·Journal of the National Cancer Institute·Haojie LiJing Ma
Apr 25, 2006·Biological Trace Element Research·Eric O UthusCindy D Davis
Apr 24, 2007·The Journal of Nutrition·Eric O Uthus, Sharon A Ross

❮ Previous
Next ❯

Citations

Aug 28, 2012·European Journal of Nutrition·Lawrence N BarreraNigel J Belshaw
Mar 16, 2011·Acta Physiologica·J A McKay, J C Mathers
Mar 6, 2012·Current Colorectal Cancer Reports·Michael Schnekenburger, Marc Diederich
Nov 4, 2017·International Journal of Food Sciences and Nutrition·Arianna Sasso, Giovanni Latella
Oct 19, 2019·British Journal of Pharmacology·Megan BeetchBarbara Stefanska
Nov 28, 2020·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Zhuo-Zhi LiangZe-Fang Ren
Nov 14, 2021·Environmental Science and Pollution Research International·Zhuo-Zhi LiangZe-Fang Ren

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.