The WAVE complex associates with sites of saddle membrane curvature.

The Journal of Cell Biology
Anne PipathsoukOrion D Weiner

Abstract

How local interactions of actin regulators yield large-scale organization of cell shape and movement is not well understood. Here we investigate how the WAVE complex organizes sheet-like lamellipodia. Using super-resolution microscopy, we find that the WAVE complex forms actin-independent 230-nm-wide rings that localize to regions of saddle membrane curvature. This pattern of enrichment could explain several emergent cell behaviors, such as expanding and self-straightening lamellipodia and the ability of endothelial cells to recognize and seal transcellular holes. The WAVE complex recruits IRSp53 to sites of saddle curvature but does not depend on IRSp53 for its own localization. Although the WAVE complex stimulates actin nucleation via the Arp2/3 complex, sheet-like protrusions are still observed in ARP2-null, but not WAVE complex-null, cells. Therefore, the WAVE complex has additional roles in cell morphogenesis beyond Arp2/3 complex activation. Our work defines organizing principles of the WAVE complex lamellipodial template and suggests how feedback between cell shape and actin regulators instructs cell morphogenesis.

References

Jan 1, 1996·Journal of Structural Biology·J R KremerJ R McIntosh
Feb 7, 1998·Science·A Hall
Jan 27, 1998·Journal of Structural Biology·D N Mastronarde
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·R D MullinsT D Pollard
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·L M MacheskyT D Pollard
Aug 31, 1999·Biophysical Journal·V C AbrahamF Lanni
Nov 7, 2001·Current Biology : CB·S KrugmannA Hall
Feb 21, 2002·The Journal of Cell Biology·Jennifer A ZallenEyal D Schejter
Aug 16, 2002·Nature·Sharon EdenMarc W Kirschner
Dec 13, 2002·Nature·Sandrine Etienne-Manneville, Alan Hall
Dec 14, 2002·Developmental Cell·Makoto KinoshitaTimothy J Mitchison
Jan 9, 2003·Current Opinion in Cell Biology·Alissa M WeaverJohn A Cooper
Feb 26, 2003·Cell·Thomas D Pollard, Gary G Borisy
Mar 26, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Marie-France CarlierDominique Pantaloni
Feb 7, 2004·The EMBO Journal·Anika SteffenTheresia E B Stradal
Mar 30, 2004·Nature Cell Biology·Metello InnocentiGiorgio Scita
Apr 27, 2004·Nature Cell Biology·Tsukasa OikawaTadaomi Takenawa
Jan 18, 2005·Current Opinion in Plant Biology·Daniel B Szymanski
Jan 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yan LengKatherine Siminovitch
Feb 3, 2005·The Journal of Cell Biology·Hideki YamaguchiJohn Condeelis
Jun 30, 2005·Journal of Cell Science·Stefanie BeneschKlemens Rottner
May 17, 2006·The Journal of Cell Biology·Shiro SuetsuguTadaomi Takenawa
Dec 22, 2006·Nature Reviews. Molecular Cell Biology·Tadaomi Takenawa, Shiro Suetsugu
Mar 21, 2007·The Journal of Cell Biology·Pieta K MattilaPekka Lappalainen
Aug 19, 2007·PLoS Biology·Orion D WeinerMarc W Kirschner
Jan 25, 2008·Trends in Cell Biology·Giorgio ScitaShiro Suetsugu
May 24, 2008·Nature·Kinneret KerenJulie A Theriot
Jul 4, 2008·Current Opinion in Cell Biology·Christopher V Carman, Timothy A Springer
Jan 27, 2009·Current Opinion in Cell Biology·Melissa A Chesarone, Bruce L Goode

❮ Previous
Next ❯

Citations

Oct 8, 2021·Nature Cell Biology·Paulina Moreno-LaysecaJohanna Ivaska
Nov 6, 2021·Nature Cell Biology·Laura M Machesky, Robert H Insall

❮ Previous
Next ❯

Software Mentioned

IMOD
MATLAB
Fiji
eTomo
Insight3
Airyscan
CUDA
ChimeraX
MiSeq
Python

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.