The WD40 Protein BamB Mediates Coupling of BAM Complexes into Assembly Precincts in the Bacterial Outer Membrane

Cell Reports
Sachith D GunasingheTrevor Lithgow

Abstract

The β-barrel assembly machinery (BAM) complex is essential for localization of surface proteins on bacterial cells, but the mechanism by which it functions is unclear. We developed a direct stochastic optical reconstruction microscopy (dSTORM) methodology to view the BAM complex in situ. Single-cell analysis showed that discrete membrane precincts housing several BAM complexes are distributed across the E. coli surface, with a nearest neighbor distance of ∼200 nm. The auxiliary lipoprotein subunit BamB was crucial for this spatial distribution, and in situ crosslinking shows that BamB makes intimate contacts with BamA and BamB in neighboring BAM complexes within the precinct. The BAM complex precincts swell when outer membrane protein synthesis is maximal, visual proof that the precincts are active in protein assembly. This nanoscale interrogation of the BAM complex in situ suggests a model whereby bacterial outer membranes contain highly organized assembly precincts to drive integral protein assembly.

Citations

Oct 28, 2019·Nature·Anatol LutherDaniel Obrecht
Jun 6, 2020·The Journal of Biological Chemistry·Jim E HorneSheena E Radford
Jul 28, 2020·Nature Reviews. Microbiology·Chris Greening, Trevor Lithgow
Sep 13, 2020·Scientific Reports·Sushmitha Vijaya KumarJennifer L Morrell-Falvey
May 3, 2020·Nature Communications·Antonio N CalabreseSheena E Radford
Dec 15, 2020·Molecular Microbiology·Karl LundquistNicholas Noinaj
Dec 16, 2020·Communications Biology·Matthew G IadanzaNeil A Ranson
Feb 10, 2021·Current Opinion in Microbiology·David Tomasek, Daniel Kahne
Feb 28, 2021·Biochimica Et Biophysica Acta. Biomembranes·Xiaoyu ChenHsin-Hui Shen
Feb 28, 2021·Journal of Molecular Biology·Kathryn A DiederichsIstvan Botos
Mar 7, 2021·International Journal of Molecular Sciences·Elisa ConsoliTanneke den Blaauwen
May 12, 2021·ACS Applied Materials & Interfaces·Sara GhavamianVictor J Cadarso
Jun 15, 2019·Microbiology Spectrum·Wolfram R Zückert
Mar 15, 2019·EcoSal Plus·Dante P Ricci, Thomas J Silhavy
Jul 9, 2021·Nature Communications·Paul WhiteSheena E Radford
Oct 31, 2021·Proceedings of the National Academy of Sciences of the United States of America·Georgina BennBart W Hoogenboom

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