The wheat resistance gene Lr34 results in the constitutive induction of multiple defense pathways in transgenic barley

The Plant Journal : for Cell and Molecular Biology
Harsh ChauhanBeat Keller

Abstract

The wheat gene Lr34 encodes an ABCG-type transporter which provides durable resistance against multiple pathogens. Lr34 is functional as a transgene in barley, but its mode of action has remained largely unknown both in wheat and barley. Here we studied gene expression in uninfected barley lines transgenic for Lr34. Genes from multiple defense pathways contributing to basal and inducible disease resistance were constitutively active in seedlings and mature leaves. In addition, the hormones jasmonic acid and salicylic acid were induced to high levels, and increased levels of lignin as well as hordatines were observed. These results demonstrate a strong, constitutive re-programming of metabolism by Lr34. The resistant Lr34 allele (Lr34res) encodes a protein that differs by two amino acid polymorphisms from the susceptible Lr34sus allele. The deletion of a single phenylalanine residue in Lr34sus was sufficient to induce the characteristic Lr34-based responses. Combination of Lr34res and Lr34sus in the same plant resulted in a reduction of Lr34res expression by 8- to 20-fold when the low-expressing Lr34res line BG8 was used as a parent. Crosses with the high-expressing Lr34res line BG9 resulted in an increase of Lr34sus expression ...Continue Reading

References

Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D AlexanderE Ward
Mar 4, 1998·Plant Molecular Biology·A B ChristensenD B Collinge
Feb 8, 2002·Trends in Plant Science·Martin Heil, Ian T Baldwin
Jan 1, 1996·Annual Review of Phytopathology·J A Kolmer
Jun 16, 2005·Plant Physiology·Kevin A T SilversteinKathryn A VandenBosch
Jun 21, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·W SpielmeyerE S Lagudah
Sep 30, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·E S LagudahW Spielmeyer
Nov 17, 2006·Nature·Jonathan D G Jones, Jeffery L Dangl
Feb 27, 2008·Trends in Plant Science·Paul J VerrierFrederica L Theodoulou
Mar 19, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M LillemoA Bjørnstad
Aug 5, 2008·Plant Physiology and Biochemistry : PPB·Jan SelsMiguel F C De Bolle
Jul 7, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Evans S LagudahBeat Keller
Jul 9, 2009·International Journal of Plant Genomics·Goetz HenselJochen Kumlehn
Aug 4, 2009·Plant Signaling & Behavior·Nazmul H BhuiyanJohn King
Mar 31, 2010·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Abdulsalam DakouriSylvie Cloutier
Jun 17, 2010·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Colin W HiebertWolfgang Spielmeyer
Sep 18, 2010·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Sybil A Herrera-FoesselRavi P Singh
Jul 12, 2011·Molecular Plant·Dong Wang, Xinnian Dong
Feb 4, 2012·The Arabidopsis Book·Joohyun KangEnrico Martinoia
Feb 11, 2012·Plant Biotechnology Journal·Joanna M RiskBeat Keller
Aug 24, 2012·Plant, Cell & Environment·Guillaume MartiJean-Luc Wolfender
Nov 3, 2012·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Simon G KrattingerEvans S Lagudah
Nov 10, 2012·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Morten LillemoRavi P Singh
May 29, 2013·Plant Biotechnology Journal·Joanna M RiskBeat Keller
Sep 24, 2013·Methods in Molecular Biology·Gaetan GlauserDirk Balmer
Oct 1, 1991·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·R P SinghS Rajaram
Feb 28, 2014·Frontiers in Plant Science·Clemencia M RojasKirankumar S Mysore

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Citations

May 24, 2016·Seminars in Cell & Developmental Biology·Elizabeth FrenchAnjali S Iyer-Pascuzzi
Sep 18, 2016·Rice·Zhijian LiangQinghua Pan
Sep 7, 2016·Scientific Reports·Nicolas Rispail, Diego Rubiales
Mar 17, 2017·Plant Biotechnology Journal·Wendelin SchnippenkoetterEvans Lagudah
Apr 22, 2017·Phytopathology·Meixiang Zhang, Gitta Coaker
Oct 18, 2018·The Journal of Biological Chemistry·Johannes P DeppeRosa L Lopéz-Marqués
Aug 11, 2017·Molecular Plant-microbe Interactions : MPMI·Denise Pereira TorresRalph Hückelhoven
Dec 7, 2019·F1000Research·Anuj SharmaFrank F White
Aug 4, 2020·Plant Biotechnology Journal·M Asyraf Md HattaBrande B H Wulff
Mar 4, 2021·Plant Biotechnology Journal·Wendy J LyzengaSateesh Kagale
Jul 12, 2021·Plant Physiology and Biochemistry : PPB·Stephanie BräunlichBeat Keller

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