The wheat transcription factor TaGAMyb recruits histone acetyltransferase and activates the expression of a high-molecular-weight glutenin subunit gene

The Plant Journal : for Cell and Molecular Biology
Weiwei GuoYingyin Yao

Abstract

Glutenin proteins in wheat (Triticum aestivum L.) flour confer unique viscoelastic properties to dough products and, therefore, the concentration and composition of the glutenin proteins determine its end-use value. However, the mechanisms governing the glutenin gene expression remain elusive. In this study, we report that wheat TaGAMyb activates the high-molecular-weight glutenin subunit genes (TaGLU) through recruiting the histone acetyltransferase GCN5. By sequencing the promoters of TaGLU-1 genes from 40 modern wheat cultivars, we identified eight types of TaGAMyb binding motifs and verified these by electrophoretic mobility shift assays. The number of TaGAMyb binding motifs in TaGLU-1 genes is correlated with the abundance of glutenin in different cultivars. Chromatin immunoprecipitation plus polymerase chain reaction (ChIP-PCR) analysis reveals that TaGCN5 directly targets the promoters of TaGLU-1 genes in wheat endosperm. We find that TaGAMyb physically interacts with the wheat histone acetyltransferase TaGCN5 and also interacts with Arabidopsis thaliana AtGCN5. TaGAMyb ectopically expressed in Arabidopsis binds to the TaGLU-1Dy promoter on a TaGLU-1Dy transgene and activates its expression. AtGCN5 also targets the TaGLU...Continue Reading

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Citations

Nov 18, 2018·The Plant Journal : for Cell and Molecular Biology·Julie BoudetCatherine Ravel
Dec 11, 2019·Plant Molecular Biology·Luning DuanXiaohui Li
Dec 8, 2017·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Jiantang ZhuGuangmin Xia
May 10, 2020·International Journal of Molecular Sciences·Dandan GuoBaoyun Li
May 22, 2019·International Journal of Molecular Sciences·Claus Krogh Madsen, Henrik Brinch-Pedersen
Feb 6, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Daowen WangKunpu Zhang
Jul 14, 2017·Frontiers in Plant Science·Fusheng SunGuangyuan He
Dec 12, 2020·Plant Biotechnology Journal·Lisha ShenAimin Zhang
Aug 4, 2020·Biochimica Et Biophysica Acta. Gene Regulatory Mechanisms·Klaus D GrasserFredy Barneche
Sep 8, 2021·The Plant Journal : for Cell and Molecular Biology·Jihu LiShuanghe Cao
Oct 12, 2021·The Plant Journal : for Cell and Molecular Biology·Guangbin LuoAimin Zhang

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