PMID: 11904217Mar 21, 2002Paper

The wild type bacterial Co(2+)/Co(2+)-phosphotriesterase shows a middle-range thermostability

Biochimica Et Biophysica Acta
D RochuPatrick Masson

Abstract

The phosphotriesterase (PTE) from Pseudomonas diminuta, a metalloenzyme that catalyses the hydrolysis of organophosphorus pesticides and nerve agents, has been described as a remarkably heat-stable protein [Grimsley et al., Biochemistry 36 (1997), 14366-14374]. Because substitution of the naturally occurring zinc ions by cobalt ions was found to enhance the enzyme catalytic activity, we investigated the thermal stability of the Co(2+)/Co(2+)-PTE. This study, carried out using capillary electrophoresis under optimised conditions in the pH range 9-10 compatible with optimal enzyme activity, provided evidence for irreversible denaturation according to the Lumry-Eyring model. A temperature-induced conformational transition (T(m) approximately equal to 58 degrees C) and an early growing of aggregates were observed. Comparison of UV spectra with heat-induced inactivation data clearly demonstrated that the PTE state populated above T(m) was neither native nor active. Differential scanning calorimetry showed only an exothermic trace due to aggregation of the denatured protein at T=76 degrees C. Accordingly, the temperature-induced denaturation process of the PTE could be described by a consecutive reaction model, including formation of...Continue Reading

References

Jul 1, 1976·Applied and Environmental Microbiology·D M Munnecke
Feb 15, 1990·Archives of Biochemistry and Biophysics·D P DumasJ R Wild
Jan 1, 1990·Annual Review of Biophysics and Biophysical Chemistry·E FreireJ M Sanchez-Ruiz
May 1, 1988·Journal of Bacteriology·C S McDanielJ R Wild
Nov 1, 1987·Biopolymers·W J Becktel, J A Schellman
Mar 15, 1967·Biochimica Et Biophysica Acta·R S Holmes, C J Masters
Feb 15, 1980·Journal of Molecular Biology·T E Creighton
Jan 1, 1995·Sub-cellular Biochemistry·J M Sanchez-Ruiz
Mar 6, 1998·Trends in Biotechnology·W Chen, A Mulchandani
Jan 20, 1999·Biochimie·R ScandurraP C Engel
Mar 20, 1999·Biochimica Et Biophysica Acta·J W TannerP A Liebman
Apr 25, 2000·Protein Engineering·S KumarR Nussinov
Apr 11, 2001·Trends in Biochemical Sciences·R M DanielR Eisenthal

❮ Previous
Next ❯

Citations

Jun 21, 2011·Toxicology Letters·Marie Trovaslet-LeroyFlorian Nachon
Nov 28, 2012·Advanced Healthcare Materials·Kheireddine El-BoubbouChristopher C Landry
Jun 29, 2011·Chembiochem : a European Journal of Chemical Biology·Peter James Baker, Jin Kim Montclare
Aug 18, 2017·Molecular BioSystems·Andrew J OlsenJin Kim Montclare
Jun 25, 2009·Journal of Enzyme Inhibition and Medicinal Chemistry·Eugénie CarlettiFlorian Nachon
Dec 23, 2009·Inorganic Chemistry·Dimitrios MaganasEdgar J J Groenen
Sep 25, 2003·Biochemistry·Wojciech DzwolakRoland Winter

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.