The Wnt segment polarity pathway and p24 protein TMED2 interact via a lectin- and decoy-type mechanism

BioRxiv : the Preprint Server for Biology
Klaus Fiedler

Abstract

The Sec14-like protein (280-385 GOLD-domain) in this study scores highly with the carbohydrate binding module (CBM) of the RetS domain from Pseudomonas aeruginosa. The thereupon modeled Cricetulus griseus p24-GOLD domain of p24, a member of intra-Golgi cargo receptors, is shown to interact with Wnt8 (wingless 8) of Xenopus laevis with a ΔG=-18.3 kcal/mol. Lower ranked models listed a smaller ΔG (PDBePISA) and energy of DelPhi interaction. Complex/hybrid N-glycans provide increasing energy of binding up to -7.1 kcal/mol to simulated p24-GOLD-ligand interaction. It is likely, that Wnt proteins and p24 cargo-receptors interact analogously to Wnt-Frizzled and that Wnt transport may involve early lectin binding. The possibly promiscuous interaction of p24-GOLD with ligands, including collagen, may shed light on cargo-receptor mediated traffic.

Related Concepts

Chinese Hamster
Biochemical Pathway
Delphi Technique
SEC14L1
Pseudomonas aeruginosa (antigen)
Golgi Apparatus
TMED2
TMED2 protein, human
Psychological Therapies
FZD1

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

© 2020 Meta ULC. All rights reserved