The Wnt segment polarity pathway and p24 protein TMED2 interact via a lectin- and decoy-type mechanism

BioRxiv : the Preprint Server for Biology
Klaus Fiedler


The Sec14-like protein (280-385 GOLD-domain) in this study scores highly with the carbohydrate binding module (CBM) of the RetS domain from Pseudomonas aeruginosa. The thereupon modeled Cricetulus griseus p24-GOLD domain of p24, a member of intra-Golgi cargo receptors, is shown to interact with Wnt8 (wingless 8) of Xenopus laevis with a ΔG=-18.3 kcal/mol. Lower ranked models listed a smaller ΔG (PDBePISA) and energy of DelPhi interaction. Complex/hybrid N-glycans provide increasing energy of binding up to -7.1 kcal/mol to simulated p24-GOLD-ligand interaction. It is likely, that Wnt proteins and p24 cargo-receptors interact analogously to Wnt-Frizzled and that Wnt transport may involve early lectin binding. The possibly promiscuous interaction of p24-GOLD with ligands, including collagen, may shed light on cargo-receptor mediated traffic.

Related Concepts

Chinese Hamster
Biochemical Pathway
Delphi Technique
Pseudomonas aeruginosa (antigen)
Golgi Apparatus
TMED2 protein, human
Psychological Therapies

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