The world of archaea: genome analysis, evolution and thermostable enzymes

Gene
S FujiwaraTadayuki Imanaka

Abstract

Pyrococcus sp. KOD1 is a newly isolated hyperthermophilic archaeon from a solfatara at a wharf on Kodakara Island, Kagoshima, Japan. A physical map of the KOD1 chromosome was constructed using pulsed-field gel electrophoresis of restriction fragments generated by AscI, PacI and PmeI. The order of the AscI fragments was deduced from Southern hybridization using the AscI, PmeI and PacI fragments as a probe. The derived physical map indicates that KOD1 possesses a circular-form genome and its size was estimated to be 2036 kb. Several cloned genes were hybridized to restriction fragments to locate their positions on the physical map. Some genes involved in the central dogma were located on the restricted segment of the genome. Novel characteristics of KOD1 enzymes are also introduced in this article.

References

Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·C R Woese, G E Fox
Feb 1, 1991·Molecular Microbiology·J Sitzmann, A Klein
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C R WoeseM L Wheelis
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J P GogartenT Oshima
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·N IwabeT Miyata
Jun 12, 1987·Science·C L SmithC R Cantor
Feb 25, 1995·Nucleic Acids Research·C OuzonisC Sander
Oct 15, 1994·FEMS Microbiology Letters·A I Bolstad
Jul 1, 1993·Nucleic Acids Research·M KrögerP Rice

❮ Previous
Next ❯

Citations

Apr 5, 2003·Bioresource Technology·G D Haki, S K Rakshit
Jun 1, 2002·Current Opinion in Chemical Biology·Costanzo Bertoldo, Garabed Antranikian
Oct 20, 2005·Journal of Bioscience and Bioengineering·Shinsuke Fujiwara
Oct 20, 2005·Journal of Bioscience and Bioengineering·S J JeonT Imanaka
Nov 25, 2000·Clinical and Experimental Immunology·A Fischer
Jul 12, 2002·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Tadayuki Imanaka, Haruyuki Atomi
Nov 16, 2004·Biochemical and Biophysical Research Communications·Shinsuke FujiwaraEi-Ichiro Fukusaki
May 18, 1999·Biochemical and Biophysical Research Communications·H HigashibataT Imanaka
May 11, 2005·Biochemical and Biophysical Research Communications·Masood Ahmed SiddiquiShinsuke Fujiwara
Mar 30, 2002·The Journal of Biological Chemistry·Hee-Seob LeeKwan-Hwa Park
Nov 15, 1997·Applied and Environmental Microbiology·M TakagiT Imanaka

❮ Previous
Next ❯