The wound response in fresh-cut lettuce involves programmed cell death events

Protoplasma
Elena T Iakimova, Ernst J Woltering

Abstract

In this work, the involvement of programmed cell death (PCD) in the wound-induced postharvest browning disorder and senescence in butterhead lettuce (Lactuca sativa L.) fresh-cuts was studied. At the wounded (cut, bruised) sites, rapid browning, loss of chlorophyll and massive cell death, accompanied with accumulation of reactive oxygen species and increased electrolyte leakage occurred in a narrow strip of tissue adjacent the injury. The dead cell morphology (protoplast and nuclei shrinkage) together with the biochemical and physiological changes resembled necrotic PCD type. With a slight delay post-wounding, senescence associated with similar cell death features was initiated in distant non-wounded sites. In addition to necrotic PCD, both in wounded and senescing tissue, the appearance of empty cell corpses was observed, indicating that part of the cells might undergo vacuolar PCD (self-digestion of cellular content after vacuole collapse). The wounding-induced local cell death at the primary site of damage suggested that PCD may serve as a mechanism to seal-off the wound by building a physical barrier of dead cells. However, the cell death at sites remote from the wound suggests the distribution of long-distance senescence-i...Continue Reading

References

Jun 29, 2000·Trends in Plant Science·B F QuirinoR M Amasino
Feb 22, 2001·Journal of Experimental Botany·J LeónJ J Sánchez-Serrano
May 2, 2002·Physiologia Plantarum·Reinaldo Campos-Vargas, Mikal E Saltveit
May 9, 2002·Journal of Experimental Botany·Steven J NeillJohn T Hancock
Jul 1, 1997·The Plant Cell·R. I. Pennell, C. Lamb
Feb 25, 2003·Journal of Experimental Botany·Simon A CoupeJocelyn R Eason
Apr 9, 2004·Nature Reviews. Molecular Cell Biology·Eric Lam
Sep 14, 2004·Journal of Experimental Botany·Wouter G van Doorn, Ernst J Woltering
Mar 8, 2005·Trends in Plant Science·Wouter G van Doorn, Ernst J Woltering
Mar 15, 2006·Molecular Plant-microbe Interactions : MPMI·Akinori KibaYasufumi Hikichi
Nov 3, 2006·Plant Physiology and Biochemistry : PPB·E T YakimovaE J Woltering
Dec 7, 2006·Plant Biotechnology Journal·Vicky Buchanan-WollastonDavid Pink
Dec 21, 2006·Annual Review of Plant Biology·Pyung Ok LimHong Gil Nam
Dec 15, 2007·Journal of Experimental Botany·Luis A J MurElena Prats
Feb 8, 2008·Journal of Experimental Botany·Theresa J ReapePaul F McCabe
Mar 4, 2008·Journal of Experimental Botany·Wouter G van Doorn, Ernst J Woltering
Mar 1, 2008·Plant Signaling & Behavior·Alessandra TisiAlessandra Cona
Mar 6, 2010·Trends in Plant Science·Ernst J Woltering
Jul 2, 2010·Plant Signaling & Behavior·John F TookerGary W Felton
Jul 14, 2010·Trends in Plant Science·Wouter G van Doorn, Ernst J Woltering
Sep 28, 2010·Plant Physiology and Biochemistry : PPB·Sarvajeet Singh Gill, Narendra Tuteja
Apr 16, 2011·Cell Death and Differentiation·W G van DoornP V Bozhkov
Apr 3, 2012·Progress in Lipid Research·S Courtney Frasch, Donna L Bratton
Aug 21, 2013·The New Phytologist·Fuqiang CuiKirk Overmyer
Oct 15, 2013·The New Phytologist·Paul F McCabe
May 7, 2014·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·P V MahajanM Geyer
Feb 28, 2016·Plant, Cell & Environment·Li Hong ZhouBarbara G Pickard
Oct 30, 2016·Journal of Experimental Botany·Kenichi ShibuyaKazuo Ichimura
May 17, 2017·Journal of Agricultural and Food Chemistry·Carlos J GarcíaFrancisco A Tomas-Barberan

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