The X-Linked Inhibitor of Apoptosis Protein Inhibitor Embelin Suppresses Inflammation and Bone Erosion in Collagen Antibody Induced Arthritis Mice

Mediators of Inflammation
Anak A S S K DharmapatniDavid R Haynes

Abstract

To investigate the effect of Embelin, an inhibitor of X-Linked Inhibitor of Apoptosis Protein (XIAP), on inflammation and bone erosion in a collagen antibody induced arthritis (CAIA) in mice. Four groups of mice (n = 6 per group) were allocated: CAIA untreated mice, CAIA treated with Prednisolone (10 mg/kg/day), CAIA treated with low dose Embelin (30 mg/kg/day), and CAIA treated with high dose Embelin (50 mg/kg/day). Joint inflammation was evaluated using clinical paw score and histological assessments. Bone erosion was assessed using micro-CT, tartrate resistant acid phosphatase (TRAP) staining, and serum carboxy-terminal collagen crosslinks (CTX-1) ELISA. Immunohistochemistry was used to detect XIAP protein. TUNEL was performed to identify apoptotic cells. Low dose, but not high dose Embelin, suppressed inflammation as reflected by lower paw scores (P < 0.05) and lower histological scores for inflammation. Low dose Embelin reduced serum CTX-1 (P < 0.05) and demonstrated lower histological score and TRAP counting, and slightly higher bone volume as compared to CAIA untreated mice. XIAP expression was not reduced but TUNEL positive cells were more abundant in Embelin treated CAIA mice. Low dose Embelin suppressed inflammation a...Continue Reading

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Citations

Oct 8, 2016·Immunology and Cell Biology·Sachin SharmaSubhrajit Biswas
Mar 2, 2017·International Journal of Molecular Sciences·Charles J Malemud
Nov 23, 2017·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Bonnie WilliamsTania Crotti
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Jul 24, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Dong WangPeng Xu
May 1, 2021·Biomolecules·Mary SpeirKate E Lawlor
Nov 30, 2021·Journal of Biochemical and Molecular Toxicology·Uzini Devi DaimaryAjaikumar B Kunnumakkara

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA

Software Mentioned

KC4
SkyScan
SPSS
Dataviewer
CTAn
NRecon

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

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