PMID: 8605629Mar 1, 1996Paper

The X-ray structures of two mutant crystallin domains shed light on the evolution of multi-domain proteins

Nature Structural Biology
B V NorledgeH P Driessen

Abstract

We use protein engineering and crystallography to simulate aspects of the early evolution of beta gamma-crystallins by observing how a single domain oligomerizes in response to changes in a sequence extension. The crystal structure of the C-terminal domain of gamma beta-crystallin with its four-residue C-terminal extension shows that the domain does not form a symmetric homodimer analogous to the two-domain pairing in beta gamma-crystallins. Instead the C-terminal extension now forms heterologous interactions with other domains leading to the solvent exposure of the natural hydrophobic interface with a consequent loss in protein solubility. However, this domain truncated by just the C-terminal tyrosine forms a symmetric homodimer of domains in the crystal lattice.

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Citations

Aug 1, 1997·Protein Science : a Publication of the Protein Society·B V NorledgeC Slingsby
Jul 9, 1998·Protein Science : a Publication of the Protein Society·G WrightC Slingsby
Jul 9, 1998·Protein Science : a Publication of the Protein Society·M HülsmeyerD Schomburg
Apr 4, 2006·Amino Acids·W HoehenwarterP R Jungblut
Aug 11, 2004·Progress in Biophysics and Molecular Biology·Hans BloemendalAnnette Tardieu
Mar 31, 1999·Progress in Biophysics and Molecular Biology·R Jaenicke
Dec 3, 1999·Progress in Biophysics and Molecular Biology·G D'Alessio
Jul 3, 1998·International Journal of Biological Macromolecules·J A Carver, R A Lindner
Apr 27, 1999·Progress in Retinal and Eye Research·J A Carver
Aug 1, 1996·Nature Structural Biology
Jun 27, 2002·European Journal of Biochemistry·Giuseppe D'Alessio
Dec 3, 1999·European Journal of Biochemistry·G D'alessio
Aug 21, 2010·Journal of Molecular Evolution·Guido KappéNicolette H Lubsen
Jul 28, 2005·Protein Science : a Publication of the Protein Society·Shannon L FlaughJonathan King
Jan 11, 2000·Eye·C Slingsby, N J Clout
Nov 14, 2001·The Journal of Biological Chemistry·Andrew G PurkissChristine Slingsby
Jun 27, 1998·Journal of Molecular Biology·S PalmeC Slingsby

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