PMID: 7541911Jun 25, 1995Paper

The Xenopus 9 bp ribosomal terminator (T3 box) is a pause signal for the RNA polymerase I elongation complex

Nucleic Acids Research
P Labhart

Abstract

In Xenopus, termination by RNA polymerase I (pol I) is mediated by the 9 bp sequence GACTTGCNC and RNA 3'-ends are formed -15-20 nt upstream of this terminator element. Here I show that this 9 bp element, also called the 'T3 box', is a pause signal for the elongating transcription complex. The two major transcripts in the paused complex have 3'-ends mapping to 15 and 21 nt upstream of the T3 box, remain bound to the template in 0.25% Sarkosyl, are subject to pyrophosphorolysis and can be chased into longer transcripts. Mutations that reduce overall termination also affect pausing, indicating that pausing is a limiting step in the termination process. Oligonucleotide competition experiments, furthermore, suggest that pausing requires a DNA binding factor. The data support a model in which the first step leading to transcription termination by pol I in Xenopus is pausing of the elongation complex upstream of the T3 box.

References

Feb 14, 1992·Science·P H von Hippel, T D Yager
Mar 1, 1989·Trends in Biochemical Sciences·N J Proudfoot
Mar 1, 1987·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R H ReederB McStay
Sep 25, 1987·Nucleic Acids Research·R H ReederP Walker
Aug 5, 1994·Science·E NudlerM Kashlev
Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·M D RuddD S Luse
Feb 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D Wang, D K Hawley
Feb 13, 2013·Molecular and Cellular Biology·Aneeshkumar G Arimbasseri, Richard J Maraia

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Citations

Jan 20, 1998·Trends in Biochemical Sciences·R H Reeder, W H Lang
Oct 25, 2012·Biochimica Et Biophysica Acta·Attila NémethHerbert Tschochner
Mar 19, 1999·Molecular and Cellular Biology·P Labhart

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