The XmnI restriction-modification system: cloning, expression, sequence organization and similarity between the R and M genes

Gene
D O NwankwoB E Slatko

Abstract

The xmnIRM genes from Xanthomonas manihotis 7AS1 have been cloned and expressed in Escherichia coli. The nucleotide (nt) sequences of both genes were determined. The XmnI methyltransferase (MTase)-encoding gene is 1861 bp in length and codes for 620 amino acids (aa) (68660 Da). The restriction endonuclease (ENase)-encoding gene is 959 bp long and therefore codes for a 319-aa protein (35275 Da). The two genes are aligned tail to tail and they overlap at their respective stop codons About 4 x 10(4) units/g wet cell paste of R.XmnI was obtained following IPTG induction in a suitable E. coli host. The xmnIR gene is expressed from the T7 promoter. M.XmnI probably modifies the first A in the sequence, GAA(N)4TTC. The xmnIR and M genes contain regions of conserved similarity and probably evolved from a common ancestor. M.XmnI is loosely related to M.EcoRI. The XmnI R-M system and the type-I R-M systems probably derived from a common ancestor.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 1, 1992·Methods in Enzymology·G G Wilson
Jan 1, 1991·Annual Review of Genetics·G G Wilson, N E Murray
Feb 1, 1991·Journal of Bacteriology·T TaoR M Blumenthal
May 25, 1991·Nucleic Acids Research·G G Wilson
Jan 1, 1990·Journal of Bacteriology·B Kearney, B J Staskawicz
Apr 25, 1990·Nucleic Acids Research·R J Roberts
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Apr 11, 1989·Nucleic Acids Research·J PósfaiR J Roberts
Aug 11, 1989·Nucleic Acids Research·L HamabletR J Roberts
Oct 1, 1987·Molecular & General Genetics : MGG·D Nwankwo, G Wilson
Apr 1, 1986·Analytical Biochemistry·E C StraussL E Hood
Dec 25, 1988·Gene·K D LunnenD O Nwankwo
Jan 1, 1987·Methods in Enzymology·K B Mullis, F A Faloona
Nov 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J HedgpethH W Boyer
Jan 1, 1980·Molecular & General Genetics : MGG·B Sain, N E Murray
Dec 20, 1980·Nucleic Acids Research·B C LinS Y Lou
May 5, 1983·Journal of Molecular Biology·J A Gough, N E Murray
Jan 11, 1984·Nucleic Acids Research·C KellerR J Roberts
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Jul 1, 1993·Nucleic Acids Research·R J Roberts, D Macelis
Feb 25, 1993·Nucleic Acids Research·H KongI Schildkraut

❮ Previous
Next ❯

Citations

Feb 12, 1998·Nucleic Acids Research·J VitkuteA Janulaitis
Jul 10, 2018·Nucleic Acids Research·Da Lin, Christopher A O'Callaghan
Nov 24, 2001·Journal of Molecular Biology·E CesnavicieneA Janulaitis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.