The XPF-ERCC1 endonuclease and homologous recombination contribute to the repair of minor groove DNA interstrand crosslinks in mammalian cells produced by the pyrrolo[2,1-c][1,4]benzodiazepine dimer SJG-136

Nucleic Acids Research
P H ClingenJ A Hartley


SJG-136, a pyrrolo[2,1-c][1,4]benzodiazepine (PBD) dimer, is a highly efficient interstrand crosslinking agent that reacts with guanine bases in a 5'-GATC-3' sequence in the DNA minor groove. SJG-136 crosslinks form rapidly and persist compared to those produced by conventional crosslinking agents such as nitrogen mustard, melphalan or cisplatin which bind in the DNA major groove. A panel of Chinese hamster ovary (CHO) cells with defined defects in specific DNA repair pathways were exposed to the bi-functional agents SJG-136 and melphalan, and to their mono-functional analogues mmy-SJG and mono-functional melphalan. SJG-136 was >100 times more cytotoxic than melphalan, and the bi-functional agents were much more cytotoxic than their respective mono-functional analogues. Cellular sensitivity of both SJG-136 and melphalan was dependent on the XPF-ERCC1 heterodimer, and homologous recombination repair factors XRCC2 and XRCC3. The relative level of sensitivity of these repair mutant cell lines to SJG-136 was, however, significantly less than with major groove crosslinking agents. In contrast to melphalan, there was no clear correlation between sensitivity to SJG-136 and crosslink unhooking capacity measured using a modified comet a...Continue Reading


Jun 11, 1996·International Journal of Cancer. Journal International Du Cancer·G DamiaM D'Incalci
Aug 17, 1996·Mutation Research·P D Lawley, D H Phillips
Mar 14, 2000·Molecular and Cellular Biology·D MuA Sancar
Jul 7, 2000·The Journal of Biological Chemistry·I KuraokaR D Wood
Aug 23, 2001·Mutation Research·M L Dronkert, R Kanaar
Feb 14, 2002·Nucleic Acids Research·Nan LiuLarry H Thompson
Mar 22, 2002·The Lancet Oncology·P J McHughJ A Hartley
Nov 13, 2002·Mutation Research·Larry H Thompson, David Schild
Mar 24, 2004·The Journal of Biological Chemistry·Nazumi Alice YamadaLarry H Thompson
May 18, 2004·Current Opinion in Cell Biology·Michael Lisby, Rodney Rothstein
Aug 17, 2004·Molecular Pharmacology·Katherine A GouldMichael J Tilby

❮ Previous
Next ❯


Dec 21, 2010·Chemical Reviews·Dyeison Antonow, David E Thurston
Mar 10, 2009·Applied and Environmental Microbiology·Wei LiBarbara Gerratana
Feb 25, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Igor PuzanovMace L Rothenberg
Apr 16, 2014·Mutation Research. Fundamental and Molecular Mechanisms of Mutagenesis·Swetha InturiRajesh Agarwal
Apr 5, 2011·Expert Opinion on Investigational Drugs·John A Hartley
Sep 3, 2010·Critical Reviews in Biochemistry and Molecular Biology·Lily C Wang, Jean Gautier
Nov 29, 2011·Toxicology Letters·Paul A JowseyPeter G Blain
May 6, 2011·Seminars in Hematology·Lorenzo M Leoni, John A Hartley
May 4, 2010·Toxicology Letters·Paul A JowseyPeter G Blain
Jan 15, 2008·Trends in Genetics : TIG·Daniel T Bergstralh, Jeff Sekelsky
Jun 15, 2010·Medicinal Research Reviews·Barbara Gerratana
Oct 16, 2012·Environmental and Molecular Mutagenesis·Shilpy Sharma, Christine E Canman
Feb 10, 2010·Environmental and Molecular Mutagenesis·Mitch McVey
Jul 27, 2010·Environmental and Molecular Mutagenesis·Richard D Wood
Dec 26, 2006·Experimental Cell Research·Peter H ClingenChristopher N Parris
Apr 29, 2014·DNA Repair·Kent W Mouw, Alan D D'Andrea
Sep 30, 2016·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Yong MaDonglu Zhang
Aug 19, 2014·PloS One·Khondaker M RahmanDavid E Thurston
Jun 20, 2019·Journal of Cellular Physiology·Sajad YaghoubiMeghdad Abdollahpour-Alitappeh
Jun 17, 2020·Expert Opinion on Biological Therapy·John A Hartley
Jul 31, 2020·Scientific Reports·Joseph A ReddyChristopher P Leamon
Dec 20, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jenny WuJohn A Hartley
May 2, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Gianmaria LiccardiDaniel Hochhauser

❮ Previous
Next ❯

Methods Mentioned


Software Mentioned

Komet Assay

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.