The XTT cell proliferation assay applied to cell layers embedded in three-dimensional matrix.

Assay and Drug Development Technologies
Lynn HuyckMarleen Van Troys

Abstract

Cell proliferation, a main target in cancer therapy, is influenced by the surrounding three-dimensional (3D) extracellular matrix (ECM). In vitro drug screening is, thus, optimally performed under conditions in which cells are grown (embedded or trapped) in dense 3D matrices, as these most closely mimic the adhesive and mechanical properties of natural ECM. Measuring cell proliferation under these conditions is, however, technically more challenging compared with two-dimensional (2D) culture and other "3D culture conditions," such as growth on top of a matrix (pseudo-3D) or in spongy scaffolds with large pore sizes. Consequently, such measurements are only slowly applied on a wider scale. To advance this, we report on the equal quality (dynamic range, background, linearity) of measuring the proliferation of cell layers embedded in dense 3D matrices (collagen, Matrigel) compared with cells in 2D culture using the easy (one-step) and in 2D well-validated, 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT)-assay. The comparison stresses the differences in proliferation kinetics and drug sensitivity of matrix-embedded cells versus 2D culture. Using the specific cell-layer-embedded 3D matrix setup, quanti...Continue Reading

References

Jun 1, 1997·Biochemical Pharmacology·K Ghoshal, S T Jacob
Jun 9, 1998·Journal of Cellular Physiology·V RantaA Orpana
Aug 6, 1998·Journal of Immunological Methods·M M NociariC Russo
Jun 6, 2000·Journal of Biomolecular Screening·J H ZhangK R Oldenburg
Feb 13, 2001·Current Opinion in Biotechnology·K Slater
Jun 19, 2001·Journal of Immunological Methods·L J JonesV L Singer
Jan 26, 2002·Medical Electron Microscopy : Official Journal of the Clinical Electron Microscopy Society of Japan·Michiko NishimuraTohru Kaku
Mar 20, 2002·Nature Reviews. Cancer·M J Bissell, D Radisky
Aug 22, 2003·Nature·Alison Abbott
Jul 28, 2006·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·A Carnero
Mar 31, 2007·Nature Methods·Genee Y LeeMina J Bissell
Aug 28, 2007·Cell·Kenneth M Yamada, Edna Cukierman
Jan 18, 2008·Biology of the Cell·Luis E Arias-Romero, Jonathan Chernoff
Jul 9, 2008·Journal of Enzyme Inhibition and Medicinal Chemistry·David ShumHakim Djaballah
Nov 28, 2008·Assay and Drug Development Technologies·Bhagwant Kaur SekhonDaniel Man-Yuen Sze
Dec 4, 2008·Drug Discovery Today·Bradley A JusticeRobin A Felder
May 26, 2009·Oncogene·P R MolliR Kumar
May 28, 2009·Biotechnology and Bioengineering·Mark W Tibbitt, Kristi S Anseth
Aug 18, 2009·Seminars in Cell & Developmental Biology·Katarina WolfPeter Friedl
Sep 8, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Irina Tsoy NizamutdinovaHye Jung Kim
Dec 3, 2009·The Journal of Cell Biology·Peter Friedl, Katarina Wolf
Jan 20, 2010·Journal of Cellular and Molecular Medicine·Verena M C QuentDietmar W Hutmacher
Mar 20, 2010·Birth Defects Research. Part C, Embryo Today : Reviews·Xiao-Dong Chen
May 11, 2010·Seminars in Cancer Biology·Edna Cukierman, Daniel E Bassi
Jun 22, 2010·Journal of Cellular Biochemistry·Fang ZhangMu-jun Zhao
Jul 28, 2010·Biochemical Society Transactions·Daniel J Maltman, Stefan A Przyborski
Aug 3, 2010·Chemical Society Reviews·Derek N Woolfson, Zahra N Mahmoud
Aug 19, 2010·Journal of Tissue Engineering and Regenerative Medicine·Loraine L Y ChiuMilica Radisic
Oct 5, 2010·The FEBS Journal·Anita AlexaAttila Reményi
Jan 7, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Amit Pathak, Sanjay Kumar
Jan 25, 2011·American Journal of Infection Control·Rachel L HulkowerMark D Sobsey

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Citations

Oct 23, 2013·Expert Opinion on Drug Discovery·Lauren KimlinVictoria Virador
Sep 2, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Devis BellucciValeria Cannillo
Feb 20, 2016·Angewandte Chemie·Falguni PatiHelene Andersson Svahn
Nov 10, 2015·Experimental Cell Research·Aleksandra SimiczyjewDorota Nowak
Sep 24, 2015·Journal of Receptor and Signal Transduction Research·Jian-Cheng XiYuan-Zheng Ma
Oct 29, 2014·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Cristina Martínez-Ramos, Myriam Lebourg
Aug 29, 2016·European Journal of Cell Biology·Maneet BhatiaKathryn F Tonissen
Feb 18, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jingzi LiuBin He
Sep 5, 2017·Clinical Hemorheology and Microcirculation·Anne-Helen LutterUrsula Anderer
Mar 8, 2018·Biomedical Microdevices·Solmaz KaramikamkarKaren C Cheung
Nov 16, 2017·Toxicological Sciences : an Official Journal of the Society of Toxicology·Xiao YuAmy L Oldenburg
Jun 4, 2019·Canadian Journal of Microbiology·Da-Seul Shin, Yong-Bin Eom
Dec 28, 2019·Methods and Protocols·Marc RuoßAndreas K Nussler
Jan 16, 2020·Medicina·Bogdan Ionel TambaTeodora Alexa-Stratulat
Oct 28, 2019·Frontiers in Pharmacology·Aleksandra SimiczyjewDorota Nowak
Mar 19, 2019·Bioinorganic Chemistry and Applications·Ayodele T Odularu, Peter A Ajibade
Jan 17, 2020·Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences·Serda Kecel-GunduzSevim Akyuz
Oct 1, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Cloé L EspositoV Gaëlle Roullin

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Methods Mentioned

BETA
FCS
Assay
ELISA

Software Mentioned

Graphpad Prism
ImageJ

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