The yajC gene from Lactobacillus buchneri and Escherichia coli and its role in ethanol tolerance

Journal of Industrial Microbiology & Biotechnology
Siqing LiuS R Hughes

Abstract

The yajC gene (Lbuc_0921) from Lactobacillus buchneri NRRL B-30929 was identified from previous proteomics analyses in response to ethanol treatment. The YajC protein expression was increased by 15-fold in response to 10 % ethanol vs 0 % ethanol. The yajC gene encodes the smaller subunit of the preprotein translocase complex, which interacts with membrane protein SecD and SecF to coordinate protein transport and secretion across cytoplasmic membrane in Escherichia coli. The YajC protein was linked to sensitivity to growth temperatures in E. coli, involved in translocation of virulence factors during Listeria infection, and stimulating a T cell-mediated response of Brucella abortus. In this study, the L. buchneri yajC gene was over-expressed in E. coli. The strain carrying pET28byajC that produces YajC after isopropyl β-D-1-thiogalactopyranoside induction showed tolerance to 4 % ethanol in growth media, compared to the control carrying pET28b. This is the first report linking YajC to ethanol stress and tolerance.

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Citations

Jul 11, 2019·Journal of Industrial Microbiology & Biotechnology·Siqing LiuNasib Qureshi
May 19, 2017·Canadian Journal of Microbiology·Jasmine M PandoJohn E Gustafson
Jul 29, 2020·Frontiers in Bioengineering and Biotechnology·Liya LiangCarrie A Eckert
Nov 7, 2020·World Journal of Microbiology & Biotechnology·Siqing LiuNasib Qureshi

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