The Yeast ATF1 Acetyltransferase Efficiently Acetylates Insect Pheromone Alcohols: Implications for the Biological Production of Moth Pheromones

Lipids
Bao-Jian DingChrister Löfstedt

Abstract

Many moth pheromones are composed of mixtures of acetates of long-chain (≥10 carbon) fatty alcohols. Moth pheromone precursors such as fatty acids and fatty alcohols can be produced in yeast by the heterologous expression of genes involved in insect pheromone production. Acetyltransferases that subsequently catalyze the formation of acetates by transfer of the acetate unit from acetyl-CoA to a fatty alcohol have been postulated in pheromone biosynthesis. However, so far no fatty alcohol acetyltransferases responsible for the production of straight chain alkyl acetate pheromone components in insects have been identified. In search for a non-insect acetyltransferase alternative, we expressed a plant-derived diacylglycerol acetyltransferase (EaDAcT) (EC 2.3.1.20) cloned from the seed of the burning bush (Euonymus alatus) in a yeast system. EaDAcT transformed various fatty alcohol insect pheromone precursors into acetates but we also found high background acetylation activities. Only one enzyme in yeast was shown to be responsible for the majority of that background activity, the acetyltransferase ATF1 (EC 2.3.1.84). We further investigated the usefulness of ATF1 for the conversion of moth pheromone alcohols into acetates in compar...Continue Reading

References

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Citations

Nov 18, 2018·FEMS Microbiology Reviews·Sylvester HoltJohan M Thevelein
Jul 18, 2017·The Plant Journal : for Cell and Molecular Biology·Tam N T TranTimothy P Durrett
Nov 23, 2016·The New Phytologist·John A Pickett, Zeyaur R Khan
Jan 26, 2021·Frontiers in Bioengineering and Biotechnology·Jaroslav HambalkoMilan Čertík
Sep 1, 2020·Current Opinion in Biotechnology·Karolis PetkeviciusIrina Borodina
Feb 26, 2021·Journal of Neurogenetics·Ross M McKinneyYehuda Ben-Shahar
Mar 4, 2021·Biotechnology Journal·Karolis PetkeviciusIrina Borodina
Oct 13, 2020·Metabolic Engineering·Carina HolkenbrinkIrina Borodina

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Methods Mentioned

BETA
acetylation
acetylate
acetylating

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