The yeast ATP binding cassette (ABC) protein genes PDR10 and PDR15 are novel targets for the Pdr1 and Pdr3 transcriptional regulators

FEBS Letters
H WolfgerK Kuchler

Abstract

The yeast transcription factors Pdr1 and Pdr3 control pleiotropic drug resistance (PDR) development, since they regulate expression of ATP-binding cassette (ABC) drug efflux pumps through binding to cis-acting sites known as PDREs (PDR responsive elements). In this report, we show by Northern blotting, gel shift mobility assays and DNase I footprinting that transcription of the ABC genes PDR10 and PDR15 is also controlled by Pdr1 and Pdr3. In addition, in vitro band shift assays demonstrate that a GST-Pdr1 fusion protein can bind to the PDREs of PDR10 and PDR15. DNase I footprinting allowed the identification of the precise PDRE binding motifs, indicating the presence of a novel slightly degenerate PDRE motif in the PDR15 promoter. Finally, PDR10 and PDR15 mRNA levels vary dramatically in abundance in isogenic yeast strains carrying either deltapdr1, deltapdr3 and deltapdr1 deltapdr3 deletions or pdr1-3 and pdr3-2 gain-of-function mutations, demonstrating that both PDR10 and PDR15 are new members of the yeast PDR network.

References

May 25, 1990·Nucleic Acids Research·M E SchmittB L Trumpower
Mar 1, 1989·Genes & Development·W S Moye-RowleyC S Parker
Jul 1, 1994·Molecular and Cellular Biology·D J KatzmannW S Moye-Rowley
Jul 5, 1996·The Journal of Biological Chemistry·J A MartensC J Brandl
Feb 1, 1997·Nature Genetics·A Decottignies, A Goffeau
Jun 3, 2010·Cold Spring Harbor Protocols·David S StrayerCarmen N Nichols

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Citations

May 20, 2009·The Journal of Membrane Biology·Nathan C RockwellJeremy Thorner
Feb 24, 2006·Applied Microbiology and Biotechnology·Ken MatsuiMitsuyoshi Ueda
Dec 7, 2010·Eukaryotic Cell·Sanjoy PaulW Scott Moye-Rowley
Aug 11, 2004·Eukaryotic Cell·Marcin KolaczkowskiW Scott Moye-Rowley
Feb 18, 2003·Molecular and Cellular Biology·Angelika KrenKarl Kuchler
Dec 1, 2009·Microbiology and Molecular Biology Reviews : MMBR·Christian M PaumiSusan Michaelis
Jun 6, 2006·Genetics·Abdul-Kader SouidW-C Winston Shen
Oct 14, 2011·Yi chuan = Hereditas·Qian Wang, Zhi-Feng Cui
Sep 7, 2014·BMC Genomics·Dominik MojzitaLaura Ruohonen
Mar 27, 2003·Molecular Microbiology·Manuela Schuetzer-MuehlbauerKarl Kuchler
Jan 13, 2006·FEBS Letters·Helmut Jungwirth, Karl Kuchler
May 7, 2009·FEMS Yeast Research·Myriam Manente, Michel Ghislain
Sep 16, 2016·Biochimica Et Biophysica Acta. General Subjects·Ken-Ichi FujitaToshio Tanaka
Aug 31, 2011·The Journal of Cell Biology·Libuse VáchováZdena Palková
May 5, 2004·The Journal of Biological Chemistry·Bassel AkacheBernard Turcotte
Jul 17, 2018·Bioscience, Biotechnology, and Biochemistry·Daisuke MiuraKatsuya Gomi
Dec 13, 2006·The Journal of Biological Chemistry·Vivienne FardeauFrédéric Devaux
Nov 16, 2019·World Journal of Microbiology & Biotechnology·Yusuf SürmeliZeynep Petek Çakar
Apr 27, 2001·The Journal of Biological Chemistry·T C HallstromW S Moye-Rowley
May 16, 2000·The Journal of Biological Chemistry·X J ChenG D Clark-Walker
Apr 12, 2002·The Journal of Biological Chemistry·Bassel Akache, Bernard Turcotte

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