The yeast prion protein Ure2 shows glutathione peroxidase activity in both native and fibrillar forms.

The Journal of Biological Chemistry
Ming BaiSarah Perrett

Abstract

Ure2p is the precursor protein of the Saccharomyces cerevisiae prion [URE3]. Ure2p shows homology to glutathione transferases but lacks typical glutathione transferase activity. A recent study found that deletion of the Ure2 gene causes increased sensitivity to heavy metal ions and oxidants, whereas prion strains show normal sensitivity. To demonstrate that protection against oxidant toxicity is an inherent property of native and prion Ure2p requires biochemical characterization of the purified protein. Here we use steady-state kinetic methods to characterize the multisubstrate peroxidase activity of Ure2p using GSH with cumene hydroperoxide, hydrogen peroxide, or tert-butyl hydroperoxide as substrates. Glutathione-dependent peroxidase activity was proportional to the Ure2p concentration and showed optima at pH 8 and 40 degrees C. Michaelis-Menten behavior with convergent straight lines in double reciprocal plots was observed. This excludes a ping-pong mechanism and implies either a rapid-equilibrium random or a steady-state ordered sequential mechanism for Ure2p, consistent with its classification as a glutathione transferase. The mutant 90Ure2, which lacks the unstructured N-terminal prion domain, showed kinetic parameters id...Continue Reading

References

Jan 1, 1980·Methods in Enzymology·K J Laidler, P S Bunting
Apr 6, 1996·Lancet·R G WillP G Smith
Mar 1, 1997·Journal of Bacteriology·S Vuilleumier
Jan 1, 1997·Chemical Research in Toxicology·R N Armstrong
Nov 14, 1997·Proceedings of the National Academy of Sciences of the United States of America·D C MasisonR B Wickner
Jul 29, 1998·Chemico-biological Interactions·B MannervikM Widersten
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·S B Prusiner
May 1, 1999·The Journal of Biological Chemistry·C ThualR Melki
Jul 2, 1999·Journal of Molecular Biology·S PerrettA R Fersht
Jan 5, 2000·Proceedings of the National Academy of Sciences of the United States of America·R ZahnK Wüthrich
Apr 28, 2000·The Journal of Biological Chemistry·P G BoardJ Pandit
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·T C UmlandD R Davies
Jul 11, 2001·The Journal of Biological Chemistry·A M Avery, S V Avery
Sep 11, 2001·Biochemical and Biophysical Research Communications·J M ZhouS Perrett
Jan 10, 2002·Journal of Molecular Biology·Despina GalaniSarah Perrett
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ulrich BaxaReed B Wickner
Feb 5, 2003·Biochemistry·Joan TorrentReinhard Lange
Apr 10, 2003·The Journal of Biological Chemistry·Emma J Collinson, Chris M Grant
Aug 12, 2003·Nature Biotechnology·Stéphane BachMarc Blondel
Nov 1, 2003·Biochemical and Biophysical Research Communications·Li ZhuSarah Perrett
Nov 20, 2003·Nature Reviews. Molecular Cell Biology·Mick F Tuite, Brian S Cox
Feb 6, 2004·The Journal of Biological Chemistry·Adrian C ApetriWitold K Surewicz

❮ Previous
Next ❯

Citations

Sep 25, 2009·Archives of Microbiology·Tatina T TodorovaAnna V Kujumdzieva
Aug 27, 2010·Archives of Microbiology·Tatina T TodorovaStéphane Vuilleumier
May 4, 2005·Journal of Structural Biology·Ulrich BaxaAlasdair C Steven
Dec 31, 2005·Nature Cell Biology·Eric D RossReed B Wickner
Jan 15, 2011·The Journal of Biological Chemistry·Yi-Qian WangSarah Perrett
Apr 11, 2008·The Journal of Biological Chemistry·Jimmy SavistchenkoRonald Melki
Mar 27, 2009·The Journal of Biological Chemistry·Zai-Rong Zhang, Sarah Perrett
Mar 27, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Li-Qiong XuSarah Perrett
Mar 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Carlos Gancedo, Carmen-Lisset Flores
Jun 16, 2005·Genetics·Nicolas TalarekMichel Aigle
Aug 11, 2012·Genetics·Susan W Liebman, Yury O Chernoff
Dec 14, 2011·International Journal of Evolutionary Biology·Anne ThuillierMélanie Morel
Jul 1, 2008·Free Radical Biology & Medicine·Ma Alba SorollaElisa Cabiscol
Feb 5, 2008·Current Opinion in Structural Biology·Sarah Perrett, Gary W Jones
Feb 9, 2008·Proteins·Daizo HamadaItaru Yanagihara
Apr 22, 2008·Protein Science : a Publication of the Protein Society·Mirko SackewitzElisabeth Schwarz
Sep 12, 2006·Journal of Molecular Biology·Frederic RousseauLaura S Itzhaki
Jan 24, 2006·Biochimica Et Biophysica Acta·Hui-Yong LianSarah Perrett
Nov 3, 2005·Current Opinion in Structural Biology·Aaron J Oakley
Jun 12, 2013·The Biochemical Journal·Kuljit SinghIpsita Roy
Nov 26, 2010·The Biochemical Journal·Yong YuSarah Perrett
Sep 23, 2011·Biochemical Society Transactions·Li-Jun ChenSarah Perrett
Jun 27, 2009·Prion·Daniel C MasisonDeepak Sharma
Oct 6, 2012·Biochimica Et Biophysica Acta·Marcel Deponte
Oct 11, 2008·Journal of Molecular Biology·Zai-Rong ZhangSarah Perrett
Nov 22, 2011·Biophysical Journal·David A BatemanReed B Wickner
Aug 24, 2010·FEMS Yeast Research·Reed B WicknerChia-Lin Winchester
Jun 2, 2016·Prion·Anton A NizhnikovIrina L Derkatch
Dec 1, 2009·Bioresource Technology·Nur Koçberber KiliçGönül Dönmez
May 26, 2017·Antioxidants & Redox Signaling·Marcel Deponte

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.