The yeast transcriptome in aerobic and hypoxic conditions: effects of hap1, rox1, rox3 and srb10 deletions

Molecular Microbiology
Manuel BecerraM E Cerdán

Abstract

The transcriptome of Saccharomyces cerevisiae was screened using the high-density membrane hybridization method, under aerobic and hypoxic conditions, in wild-type and mutant backgrounds obtained by the disruption of the genes encoding the regulatory proteins Hap1, Rox1 and the Srb10 and Rox3 subunits of RNA polymerase II holoenzyme. None of the mutations studied was able to fully overcome the wild-type hypoxic response. Deletion of the hap1 gene changed the expression profiles of individual open reading frames (ORFs) under both aerobic and hypoxic conditions. Major changes associated with rox3 deletion were related to the hypoxic activation. Rox3 also caused a repressor effect (oxygen-independent) on a subset of genes related to subtelomeric proteins. With regard to the effect brought about by the deletion of rox1 and srb10, correspondence cluster analysis revealed that the transcriptome profile in aerobic conditions is very similar in the wild-type and both deletion strains. In contrast, however, differences were found during hypoxia between the subgroup formed by wild-type and the Deltarox1 deletant compared with the Deltasrb10 deletant. An analysis of selected ORFs responding to hypoxia, in association with a dependence on ...Continue Reading

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Citations

Dec 23, 2011·Applied Microbiology and Biotechnology·Angel Vizoso-VázquezM Esperanza Cerdán
Sep 26, 2012·Biotechnology Letters·M Isabel González SisoM Esperanza Cerdán
Apr 27, 2010·Eukaryotic Cell·César M Camilo, Suely L Gomes
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Oct 30, 2007·Eukaryotic Cell·Eve LenssenMartine A Collart
Oct 23, 2004·Genome Génome / Conseil National De Recherches Canada·Manuel BecerraM Esperanza Cerdán
Jul 3, 2013·Annual Review of Microbiology·Geraldine Butler
Aug 29, 2012·Oxidative Medicine and Cellular Longevity·M Isabel González Siso, M Esperanza Cerdán
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Sep 1, 2009·Microbial Cell Factories·M Isabel González-SisoM Esperanza Cerdán
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May 6, 2019·Microbiology·Frédéric Devaux, Antonin Thiébaut

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