PMID: 8955331Dec 1, 1996Paper

The yeast two-hybrid system detects interactions between Bacillus subtilis sigmaB regulators

Journal of Bacteriology
U VoelkerW G Haldenwang

Abstract

SigmaB, the general stress response sigma factor of Bacillus subtilis, is regulated by the products of seven genes (rsbR, S, T, U, V, W, and X) with which it is cotranscribed. Biochemical techniques previously revealed physical associations among RsbW, RsbV, and sigmaB but failed to detect interactions of RsbR, S, T, U, or X with each other or RsbV, RsbW, or sigmaB. Using the yeast two-hybrid system, we have now obtained evidence for such interactions. The yeast reporter system was activated when RsbS was paired with either RsbR or RsbT, RsbR was paired with RsbT, and RsbV was paired with either RsbU or RsbW. In addition, RsbW2 and RsbR2 dimer formation was detected. RsbX failed to show interactions with itself or any of the other sigB operon products.

References

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Aug 1, 1994·Trends in Genetics : TIG·S Fields, R Sternglanz
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Citations

Jan 19, 1999·Annual Review of Microbiology·K T Hughes, K Mathee
Sep 3, 2003·Molecular Microbiology·Chien-Cheng ChenOlivier Delumeau
Jul 21, 2015·Photochemistry and Photobiology·Jeroen B van der Steen, Klaas J Hellingwerf
Sep 25, 2002·Journal of Bacteriology·Olivier DelumeauMichael D Yudkin
Dec 3, 2005·Journal of Bacteriology·Shuyu ZhangW G Haldenwang
Nov 18, 2005·Journal of Bacteriology·Maria Magdalena SennMarkus Bischoff
Mar 27, 1999·Biochemical and Biophysical Research Communications·J M ScottW G Haldenwang
Apr 29, 2006·International Journal of Medical Microbiology : IJMM·Jan Pané-FarréMichael Hecker

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