PMID: 8614637Apr 1, 1996Paper

The yeast UME6 gene is required for both negative and positive transcriptional regulation of phospholipid biosynthetic gene expression

Nucleic Acids Research
J C Jackson, J M Lopes

Abstract

In Saccharomyces cerevisiae, regulation of the phospholipid biosynthetic genes, INO1, CHO1, CHO2 and OPI3, is known to occur at the level of transcript abundance. Derepression in response to inositol deprivation requires the INO2 and INO4 regulatory genes. Repression in response to inositol supplementation requires the OPI1 regulatory gene. Here, we examined the role of the UME6 global negative regulatory gene in expression of the phospholipid biosynthetic genes. These studies were stimulated by the finding that the INO1 promoter included a UME6 cognate cis-acting regulatory sequence (URS1). We found that the UME6 negative regulatory gene was involved in regulation of phospholipid biosynthetic gene expression through two distinct regulatory pathways. One pathway was the direct repression of INO1 expression through the URS1 element. Surprisingly, the UME6 gene was also required for derepression of CHO1, CHO2 and OPI3 gene expression. Consistent with this observation, the UME6 gene was required for wild-type levels of expression of the INO2 positive regulatory gene. Therefore, the UME6 gene has both a negative and a positive role in regulating phospholipid biosynthesis.

References

May 1, 1975·Genetics·M R Culbertson, S A Henry
Jan 1, 1992·Trends in Genetics : TIG·L Guarente, O Bermingham-McDonogh
Sep 1, 1992·Molecular and Cellular Biology·A K VershonA D Johnson
Jan 1, 1992·Current Genetics·D C ChenT T Kuo
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·L E BuckinghamR E Esposito
May 1, 1989·Molecular and Cellular Biology·H O Park, E A Craig
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R StrichR E Esposito
Jan 1, 1982·Molecular & General Genetics : MGG·M L GreenbergS A Henry
Mar 1, 1981·The Journal of Pediatrics·P A CzajkaJ P Duffy
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·B P Ashburner, J M Lopes
Jun 1, 1995·Molecular and Cellular Biology·K S BowdishA P Mitchell
Jun 2, 1995·Cell·A D Johnson
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·M VidalR F Gaber
Apr 1, 1994·Genes & Development·R StrichR E Esposito
Apr 1, 1993·Molecular and Cellular Biology·K S Bowdish, A P Mitchell
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·C M Steber, R E Esposito

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Citations

Dec 3, 2005·Current Genetics·Mohan R Kaadige, John M Lopes
Jun 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Clinton R BartholomewDaniel J Klionsky
Aug 11, 1998·Microbial & Comparative Genomics·X Zhang, T F Smith
May 23, 2001·Nucleic Acids Research·B AkacheB Turcotte
Mar 10, 2001·Genes & Development·N A KentJ Mellor
Sep 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Sarah MacPhersonBernard Turcotte
Mar 7, 2012·Microbiology and Molecular Biology Reviews : MMBR·Edward Winter
Apr 4, 2006·Genetics·Leandria C HancockJohn M Lopes
Oct 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Roy M WilliamsRochelle E Esposito
May 29, 2004·Molecular Microbiology·Kyle R GardenourJohn M Lopes
Feb 6, 2015·Biological Reviews of the Cambridge Philosophical Society·Vemika ChandraPawan Gupta
Jul 21, 2006·Biochimica Et Biophysica Acta·Meng ChenJohn M Lopes
Nov 30, 2005·Biochimica Et Biophysica Acta·Michael J CarrozzaJerry L Workman
Aug 28, 2003·Archives of Biochemistry and Biophysics·Guimin GuanIshaiahu Shechter
Oct 31, 2009·Microbiology·Emily K BetheaTodd B Reynolds
Jan 15, 2014·Chemistry and Physics of Lipids·Susan A HenryStephen A Jesch
Oct 7, 2015·The EMBO Journal·Kaori SakakibaraKoji Okamoto
Apr 12, 2002·The Journal of Biological Chemistry·Bassel Akache, Bernard Turcotte
Feb 22, 2014·G3 : Genes - Genomes - Genetics·Bryan Salas-Santiago, John M Lopes
Oct 13, 2020·Nucleic Acids Research·Wanwen ZengRui Jiang

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