The Yersinia enterocolitica GsrA stress protein, involved in intracellular survival, is induced by macrophage phagocytosis.

Infection and Immunity
T YamamotoS Kamiya

Abstract

The Yersinia enterocolitica gsrA gene is a stress protein gene which was originally identified as essential for protecting cells under both extracellular environmental stress and intracellular stress in macrophages due to phagocytosis. The gsrA gene was shown to be a member of the htrA class of genes and to possess a sequence homologous to that of the promoter recognized by a stress-induced sigma factor, sigmaE. In order to study the induction of the potentially sigmaE-controlled gsrA gene in Y. enterocolitica after phagocytosis by macrophages, we identified GsrA by overproducing the protein using a T7 promoter-gsrA fusion. We found that it is translated as an unstable 49,500-Da protein which is processed by removal of an amino acid fragment consisting of 27 residues, resulting in a stable 46,800-Da protein. By radiolabeling proteins specific to bacteria in the J774-1 macrophage-like cell line, we found that the production of GsrA protein is indeed enhanced in bacterial cells growing within macrophage phagosomes. Transcriptional activation of the gsrA gene was determined by using the gsrA promoter-lacZ fusion system. This work provides the first piece of evidence that the sigmaE regulon responds to the stressful environment fou...Continue Reading

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Citations

Mar 15, 2016·Frontiers in Cellular and Infection Microbiology·Shiyun ChenMatthew S Francis
Feb 23, 1999·Molecular Microbiology·R ManganelliI Smith
Feb 28, 2004·FEMS Microbiology Letters·Gerhard HeusippVirginia L Miller
Jun 18, 2002·Infection and Immunity·Dirk BumannPeter R Jungblut
May 11, 2005·Molecular Microbiology·Tracy L Raivio
Jan 16, 2002·Journal of Bacteriology·Toshifumi TomoyasuTomoko Yamamoto
Jun 9, 2017·Future Microbiology·James W MarshWilhelmina M Huston
Mar 15, 2003·International Journal of Medical Microbiology : IJMM·Uri Gophna, Eliora Z Ron
May 25, 2001·Microbial Pathogenesis·D M Jensen-Cain, F D Quinn

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