The Yersiniabactin-Associated ATP Binding Cassette Proteins YbtP and YbtQ Enhance Escherichia coli Fitness during High-Titer Cystitis

Infection and Immunity
Eun-Ik KohJeffrey P Henderson

Abstract

The Yersinia high-pathogenicity island (HPI) is common to multiple virulence strategies used by Escherichia coli strains associated with urinary tract infection (UTI). Among the genes in this island are ybtP and ybtQ, encoding distinctive ATP binding cassette (ABC) proteins associated with iron(III)-yersiniabactin import in Yersinia pestis In this study, we compared the impact of ybtPQ on a model E. coli cystitis strain during in vitro culture and experimental murine infections. A ybtPQ-null mutant exhibited no growth defect under standard culture conditions, consistent with nonessentiality in this background. A growth defect phenotype was observed and genetically complemented in vitro during iron(III)-yersiniabactin-dependent growth. Following inoculation into the bladders of C3H/HEN and C3H/HeOuJ mice, this strain exhibited a profound, 10(6)-fold competitive infection defect in the subgroup of mice that progressed to high-titer bladder infections. These results identify a virulence role for YbtPQ in the highly inflammatory microenvironment characteristic of high-titer cystitis. The profound competitive defect may relate to the apparent selection of Yersinia HPI-positive E. coli in uncomplicated clinical UTIs.

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Citations

Jun 6, 2018·Future Microbiology·Anne E RobinsonJeffrey P Henderson
Aug 16, 2018·The Journal of Biological Chemistry·Anne E RobinsonJeffrey P Henderson
Aug 2, 2017·Nature Chemical Biology·Eun-Ik KohJeffrey P Henderson
Sep 26, 2017·The Journal of Clinical Investigation·Shannon I OhlemacherJeffrey P Henderson
Apr 16, 2020·International Journal of Molecular Sciences·Jaewoo YimSang Woo Seo
Feb 23, 2020·Science Advances·Zhiming WangHongjin Zheng

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