The yin and yang of solubilization and stabilization for wild-type and full-length membrane protein

Methods : a Companion to Methods in Enzymology
David HardyAnass Jawhari

Abstract

Membrane proteins (MP) are stable in their native lipid environment. To enable structural and functional investigations, MP need to be extracted from the membrane. This is a critical step that represents the main obstacle for MP biochemistry and structural biology. General guidelines and rules for membrane protein solubilization remain difficult to establish. This review aims to provide the reader with a comprehensive overview of the general concepts of MP solubilization and stabilization as well as recent advances in detergents innovation. Understanding how solubilization and stabilization are intimately linked is key to facilitate MP isolation toward fundamental structural and functional research as well as drug discovery applications. How to manage the tour de force of destabilizing the lipid bilayer and stabilizing MP at the same time is the holy grail of successful isolation and investigation of such a delicate and fascinating class of proteins.

Citations

May 28, 2019·Applied Microbiology and Biotechnology·Augusto Quaresma PedroLuís António Passarinha
Jul 7, 2020·Biotechnology Progress·Livia PilattiElisabeth de Fátima Pires Augusto
May 30, 2019·International Journal of Molecular Sciences·Tristan O C KwanIsabel Moraes
Dec 19, 2018·Biochemical Society Transactions·Rosana Reis, Isabel Moraes
Oct 4, 2020·Communications Biology·Nash D RochmanEugene V Koonin
Dec 12, 2020·Frontiers in Bioengineering and Biotechnology·Angela ChenGuillermo A Altenberg
Mar 12, 2021·Quarterly Reviews of Biophysics·Edoardo D'Imprima, Werner Kühlbrandt
Feb 18, 2020·Methods : a Companion to Methods in Enzymology·Clarisse FaugierAnass Jawhari
Jun 11, 2021·Biochemical Society Transactions·Lucas UngerAlice J Rothnie

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