The yjeB (nsrR) gene of Escherichia coli encodes a nitric oxide-sensitive transcriptional regulator
Abstract
Microarray studies of the Escherichia coli response to nitric oxide and nitrosative stress have suggested that additional transcriptional regulators of this response remain to be characterized. We identify here the product of the yjeB gene as a negative regulator of the transcription of the ytfE, hmpA and ygbA genes, all of which are known to be upregulated by nitrosative stress. Transcriptional fusions to the promoters of these genes were expressed constitutively in a yjeB mutant, indicating that all three are targets for repression by YjeB. An inverted repeat sequence that overlaps the -10 element of all three promoters is proposed to be a binding site for the YjeB protein. A similar inverted repeat sequence was identified in the tehA promoter, which is also known to be sensitive to nitrosative stress. The ytfE, hmpA, ygbA, and tehA promoters all caused derepression of a ytfE-lacZ transcriptional fusion when present in the cell in multiple copies, presumably by a repressor titration effect, suggesting the presence of functional YjeB binding sites in these promoters. However, YjeB regulation of tehA was weak, as judged by the activity of a tehA-lacZ fusion, perhaps because YjeB repression of tehA is masked by other regulatory ...Continue Reading
References
Respiratory detoxification of nitric oxide by the cytochrome c nitrite reductase of Escherichia coli
Citations
Regulation of denitrification genes in Neisseria meningitidis by nitric oxide and the repressor NsrR
Prokaryotic genome regulation: multifactor promoters, multitarget regulators and hierarchic networks
Unresolved sources, sinks, and pathways for the recovery of enteric bacteria from nitrosative stress
The CymR regulator in complex with the enzyme CysK controls cysteine metabolism in Bacillus subtilis
Interplay between enterohaemorrhagic Escherichia coli and nitric oxide during the infectious process
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