The YrdC protein--a putative ribosome maturation factor

Biochimica Et Biophysica Acta
Magdalena Kaczanowska, Monica Rydén-Aulin

Abstract

Release factor one (RF1) terminates protein synthesis in response to stop codons UAG and UAA. A mutant allele of RF1 causes temperature sensitive growth at 42 degrees C. We have earlier described the isolation of a suppressor of the temperature sensitive phenotype. The suppressor mutation is a small deletion in the open reading frame yrdC, and we have shown that the DeltayrdC mutation leads to immature 30S subunits and, as a consequence, to fewer translating ribosomes. YrdC is a small conserved protein with a dsRNA-binding surface. Here, we have characterized the YrdC protein. We show that the deletion leads to no production of functional protein, and we have indications that the YrdC protein might be essential in a wild type background. The protein is needed for the maturation of 16S rRNA, even though it does not interact tightly with either of the ribosomal subunits, or the 70S particles. The less effective maturation of rRNA affects the ribosomal feedback control, leading to an increase in expression from P1rrnB. We suggest that the function of the YrdC protein is to keep an rRNA structure needed for proper processing of 16S rRNA, especially at lower temperatures. This activity may require other factor(s). We suggest the gen...Continue Reading

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Citations

Mar 17, 2009·Nucleic Acids Research·Basma El YacoubiValérie de Crécy-Lagard
Jul 8, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Tian Min FuXiao Dong Su
Oct 18, 2006·Antimicrobial Agents and Chemotherapy·Susan M McGaha, W Scott Champney
Jun 22, 2006·Journal of Bacteriology·Florence Arsène-PloetzeFrançoise Bringel
Nov 4, 2009·Molecular and Cellular Biology·Changyi A LinHeather L True
Sep 7, 2007·Microbiology and Molecular Biology Reviews : MMBR·Magdalena Kaczanowska, Monica Rydén-Aulin
Mar 5, 2009·Microbiology and Molecular Biology Reviews : MMBR·Bruce A Maguire
Aug 10, 2011·BMC Genomics·Svetlana GerdesValérie de Crécy-Lagard
Apr 22, 2008·BMC Microbiology·Jenny FernebroBirgitta Henriques Normark
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Sep 5, 2015·Molecular Microbiology·Patrick C ThiavilleValérie de Crécy-Lagard
Jul 22, 2010·Trends in Biotechnology·Michael Y Galperin, Eugene V Koonin
Apr 21, 2009·Trends in Biochemical Sciences·Keith Connolly, Gloria Culver
Jan 20, 2009·Journal of Molecular Biology·Stefan NordP Mikael Wikström
Feb 3, 2011·The EMBO Journal·Basma El YacoubiValérie de Crécy-Lagard
Jul 11, 2006·Journal of Molecular Microbiology and Biotechnology·Koichi InoueMasayori Inouye
Mar 13, 2009·American Journal of Physiology. Cell Physiology·Olga Prokopenko, Oleg Mirochnitchenko

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