PMID: 15328602Aug 27, 2004Paper

The zinc-dependent redox switch domain of the chaperone Hsp33 has a novel fold

Journal of Molecular Biology
Hyung-Sik WonH Jane Dyson

Abstract

The Escherichia coli chaperone Hsp33 contains a C-terminal zinc-binding domain that modulates activity by a so-called "redox switch". The oxidized form in the absence of zinc is active, while the reduced form in the presence of zinc is inactive. X-ray crystal structures of Hsp33 invariably omit details of the C-terminal domain, which is truncated in protein constructs that are capable of forming crystals. We report the solution structure of a recombinant 61-residue protein containing the zinc-binding domain (residues 227-287) of Hsp33, in the presence of stoichiometric amounts of Zn2+. The zinc-bound protein is well folded, and forms a novel structure unlike other published zinc-binding domains. The structure consists of two helices at right-angles to each other, a two-stranded B-hairpin and a third helix at the C terminus. The zinc site comprises the side-chains of the conserved cysteine residues 232, 234, 262 and 265, and connects a short sequence before the first helix with the tight turn in the middle of the B-hairpin. The structure of the C-terminal zinc-binding domain suggests a mechanism for the operation of the redox switch: loss of the bound zinc ion disrupts the folded structure, allowing the ligand cysteine residues ...Continue Reading

References

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Citations

Jul 7, 2011·Quarterly Reviews of Biophysics·H Jane Dyson
Apr 17, 2009·Biochemistry·Caroline Kumsta, Ursula Jakob
May 23, 2007·Nature Structural & Molecular Biology·Marianne IlbertUrsula Jakob
Jun 15, 2006·Antioxidants & Redox Signaling·Marianne IlbertUrsula Jakob
Apr 22, 2015·The Plant Journal : for Cell and Molecular Biology·Na'ama Segal, Michal Shapira
Apr 29, 2015·Progress in Nuclear Magnetic Resonance Spectroscopy·Björn M Burmann, Sebastian Hiller
Jul 1, 2017·PloS One·Melinda S HanesRichard D Cummings
Nov 19, 2004·The Journal of Biological Chemistry·Ana Mirela NeculaiStefan Becker
Mar 15, 2005·Critical Reviews in Biochemistry and Molecular Biology·Jeannette Winter, Ursula Jakob
Sep 8, 2005·Journal of Computational Chemistry·Jianhan ChenCharles L Brooks
Jun 20, 2019·Journal of the Royal Society, Interface·Marco KrewingJulia E Bandow
Aug 1, 2016·Chemical Science·Vincent LebrunOlivier Sénèque
Aug 28, 2020·Frontiers in Microbiology·Samar AraminDana Reichmann
Oct 5, 2019·F1000Research·Camille V Goemans, Jean-François Collet
Dec 2, 2009·Journal of the American Chemical Society·Harini KaluarachchiDeborah B Zamble

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