The zinc finger E-box-binding homeobox 1 (Zeb1) promotes the conversion of mouse fibroblasts into functional neurons.

The Journal of Biological Chemistry
Long YanHongmei Wang

Abstract

The zinc finger E-box-binding transcription factor Zeb1 plays a pivotal role in the epithelial-mesenchymal transition. Numerous studies have focused on the molecular mechanisms by which Zeb1 contributes to this process. However, the functions of Zeb1 beyond the epithelial-mesenchymal transition remain largely elusive. Using a transdifferentiation system to convert mouse embryonic fibroblasts (MEFs) into functional neurons via the neuronal transcription factors achaete-scute family bHLH (basic helix-loop-helix) transcription factor1 (Ascl1), POU class 3 homeobox 2 (POU3F2/Brn2), and neurogenin 2 (Neurog2, Ngn2) (ABN), we found that Zeb1 was up-regulated during the early stages of transdifferentiation. Knocking down Zeb1 dramatically attenuated the transdifferentiation efficiency, whereas Zeb1 overexpression obviously increased the efficiency of transdifferentiation from MEFs to neurons. Interestingly, Zeb1 improved the transdifferentiation efficiency induced by even a single transcription factor (e.g. Asc1 or Ngn2). Zeb1 also rapidly promoted the maturation of induced neuron cells to functional neurons and improved the formation of neuronal patterns and electrophysiological characteristics. Induced neuron cells could form functi...Continue Reading

References

Dec 4, 2003·Gene Expression Patterns : GEP·Douglas S DarlingJoseph P Feller
Nov 6, 2007·Clinical & Experimental Metastasis·Geert BerxJonathan P Sleeman
Jan 15, 2008·Development·Yongqing LiuDouglas C Dean
Jun 10, 2008·Developmental Cell·Jing Yang, Robert A Weinberg
Dec 2, 2009·The Journal of Cell Biology·Ellen BellonPrzemko Tylzanowski
Jan 29, 2010·Nature·Thomas VierbuchenMarius Wernig
Feb 9, 2010·The Journal of Biological Chemistry·Jiejun FuHongmei Wang
Apr 27, 2011·The Journal of Experimental Medicine·Taewan KimCarlo M Croce
May 28, 2011·Nature·Zhiping P PangMarius Wernig
Jun 8, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ulrich PfistererMalin Parmar
Jul 15, 2011·Nature·Andrew S YooGerald R Crabtree
Aug 20, 2011·Cell Stem Cell·Esther Y SonKevin Eggan
Nov 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ester Sánchez-TillóAntonio Postigo
Nov 23, 2011·Cell Research·Fengxi MengJianwei Jiao
Jan 21, 2012·Histochemistry and Cell Biology·Jeong-Oh ShinHan-Sung Jung
Feb 9, 2012·F1000 Biology Reports·Archana Shenoy, Robert Blelloch
Feb 22, 2012·Cellular and Molecular Life Sciences : CMLS·Alexander GheldofGeert Berx
Apr 10, 2012·Nature Methods·Julia LadewigOliver Brüstle
Sep 21, 2013·Proceedings of the National Academy of Sciences of the United States of America·Soham ChandaThomas C Südhof
Dec 7, 2013·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Elina Pirinen, Ylermi Soini
Jan 10, 2014·Journal of Cellular and Molecular Medicine·Yu-Chao ChangWen-Wei Chang
Sep 26, 2014·Stem Cell Reports·Soham ChandaMarius Wernig

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