The zinc finger proteins ZNF644 and WIZ regulate the G9a/GLP complex for gene repression

ELife
Chunjing BianXiaochun Yu

Abstract

The G9a/GLP complex mediates mono- and dimethylation of Lys9 of histone H3 at specific gene loci, which is associated with transcriptional repression. However, the molecular mechanism by which the G9a/GLP complex is targeted to the specific gene loci for H3K9 methylation is unclear. In this study, with unbiased protein affinity purification, we found ZNF644 and WIZ as two core subunits in the G9a/GLP complex. ZNF644 and WIZ interact with the transcription activation domain of G9a and GLP, respectively. Moreover, both ZNF644 and WIZ contain multiple zinc finger motifs that recognize consensus DNA sequences. ZNF644 and WIZ target G9a and GLP to the chromatin and mediate the G9a/GLP complex-dependent H3K9 methylation as well as gene repression. Thus, our studies reveal two key subunits in the G9a/GLP complex that regulate the function of this histone methyltransferase complex.

References

Jan 1, 1987·Cold Spring Harbor Symposia on Quantitative Biology·A Klug, D Rhodes
Feb 27, 2004·Nature Immunology·Ildikó GyoryKenneth L Wright
Apr 21, 2004·Nature Genetics·Ruey-Chyi SuStephen T Smale
Jul 23, 2004·Proceedings of the National Academy of Sciences of the United States of America·Hitomi Nishio, Martin J Walsh
Aug 10, 2005·Genome Biology·Shane C DillonXiaodong Cheng
Feb 8, 2006·The Journal of Biological Chemistry·David Y LeeMichael R Stallcup
May 17, 2006·The Journal of Biological Chemistry·Jun UedaYoichi Shinkai
Nov 7, 2006·Genes & Development·Pierre-Olivier EstèveSriharsa Pradhan
Feb 10, 2007·Molecular Cell·Stefan KubicekThomas Jenuwein
Jun 30, 2007·The EMBO Journal·Makoto TachibanaYoichi Shinkai
Feb 12, 2008·Nature Structural & Molecular Biology·Robert E CollinsXiaodong Cheng
Sep 27, 2008·The EMBO Journal·Makoto TachibanaYoichi Shinkai
Oct 28, 2008·Nature Structural & Molecular Biology·Silvina Epsztejn-LitmanYehudit Bergman
Mar 10, 2009·Current Biology : CB·Feng ZhangXiaochun Yu
Feb 2, 2010·The Journal of Biological Chemistry·Jing HuangShelley L Berger
Dec 24, 2010·Nucleic Acids Research·Tao YeLaszlo Tora
Apr 19, 2011·Genes & Development·Yoichi Shinkai, Makoto Tachibana
Mar 14, 2012·The Journal of Experimental Medicine·Terry C FangAlexander Tarakhovsky
Nov 16, 2012·Proceedings of the National Academy of Sciences of the United States of America·Danielle BittencourtMichael R Stallcup
Dec 12, 2012·Nature·Qiang ChenXiaochun Yu

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Citations

Apr 6, 2018·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Sateesh MaddirevulaFowzan S Alkuraya
Jul 24, 2015·Nature Reviews. Molecular Cell Biology·Chiara MozzettaSlimane Ait-Si-Ali
Sep 5, 2015·The Journal of Biological Chemistry·Jeremy M SimonSamantha G Pattenden
Feb 6, 2017·The Journal of Biological Chemistry·Yadong WangQuan Zhao
Dec 19, 2019·Frontiers in Genetics·Victor G MartinezJesus Maria Paramio
Jul 12, 2018·Science Signaling·Yichong ZhangGerald S Shadel
May 31, 2020·Nature Chemistry·Antony J BurtonTom W Muir
Jun 10, 2017·Neurogenesis·Steven J DeimlingVincent Tropepe
Dec 31, 2020·Life Science Alliance·Baskar ChakrapaniArunkumar Dhayalan
Jul 3, 2021·International Journal of Molecular Sciences·Amanda Miles, Vincent Tropepe

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Datasets Mentioned

BETA
GSE62616

Methods Mentioned

BETA
tandem protein affinity purification
affinity purification
co-immunoprecipitation
affinity
co-IP
pull-down
ChIP
ChIP-seq
Electrophoretic
Assay

Software Mentioned

Galaxy browser
Bowtie
Cisgenome
MACS
- motifs
Peak
FastQC
SEQMINER

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