The zinc-fingers of KREPA3 are essential for the complete editing of mitochondrial mRNAs in Trypanosoma brucei.

PloS One
Xuemin GuoK Stuart

Abstract

Most mitochondrial mRNAs in trypanosomes undergo uridine insertion/deletion editing that is catalyzed by approximately 20S editosomes. The editosome component KREPA3 is essential for editosome structural integrity and its two zinc finger (ZF) motifs are essential for editing in vivo but not in vitro. KREPA3 function was further explored by examining the consequence of mutation of its N- and C-terminal ZFs (ZF1 and ZF2, respectively). Exclusively expressed myc-tagged KREPA3 with ZF2 mutation resulted in lower KREPA3 abundance and a relative increase in KREPA2 and KREL1 proteins. Detailed analysis of edited RNA products revealed the accumulation of partially edited mRNAs with less insertion editing compared to the partially edited mRNAs found in the cells with wild type KREPA3 expression. Mutation of ZF1 in TAP-tagged KREPA3 also resulted in accumulation of partially edited mRNAs that were shorter and only edited in the 3'-terminal editing region. Mutation of both ZFs essentially eliminated partially edited mRNA. The mutations did not affect gRNA abundance. These data indicate that both ZFs are essential for the progression of editing and perhaps its accuracy, which suggests that KREPA3 plays roles in the editing process via its ...Continue Reading

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Citations

Mar 8, 2011·The Journal of Biological Chemistry·Houtan MoshiriReza Salavati
Aug 1, 2015·FEBS Letters·Anna CzerwoniecJanusz M Bujnicki
Nov 18, 2015·Trends in Parasitology·Inna Aphasizheva, Ruslan Aphasizhev
Aug 9, 2011·Wiley Interdisciplinary Reviews. RNA·Ruslan Aphasizhev, Inna Aphasizheva
Dec 1, 2012·International Journal for Parasitology. Drugs and Drug Resistance·Reza SalavatiHamed Shateri Najafabadi
Mar 24, 2017·Nucleic Acids Research·Jason CarnesKenneth Stuart
Aug 14, 2018·Wiley Interdisciplinary Reviews. RNA·Jorge Cruz-ReyesShelly Gulati
May 9, 2012·Eukaryotic Cell·Melissa LerchKenneth Stuart
Aug 26, 2015·The Journal of Biological Chemistry·Suzanne M McDermottKenneth Stuart

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Methods Mentioned

BETA
PCR
co-immunoprecipitation
electrophoresis
guanylytransferase
protein exchange
X-ray

Software Mentioned

gMURF2

Related Concepts

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African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.