The zoospores of the thraustochytrid Aurantiochytrium limacinum: Transcriptional reprogramming and lipid metabolism associated to their specific functions.

Environmental Microbiology
Younès DelleroFabrice Rébeillé

Abstract

Aurantiochytrium limacinum (Thraustochytriaceae, class Labyrinthulomycetes) is a marine Stramenopile and a pioneering mangrove decomposer. Its life cycle involves a non-motile stage and zoospore production. We observed that the composition of the medium, the presence of amino acids in particular, affects the release of zoospores. Two opposite conditions were defined, one with a cell population mainly composed of zoospores and another one with almost only non-motile cells. In silico allelic frequency analysis and flow cytometry suggest that zoospores and non-motile cells share the same ploidy level and are diploid. Through an RNA-seq approach, the transcriptional reprogramming accompanying the formation of zoospores was investigated, with a particular focus on their lipid metabolism. Based on a differential expression analysis, zoospores are characterized by high motility, very active signal transduction, an arrest of the cell division, a low amino acid metabolism and low glycolysis. Focusing on lipid metabolism, genes involved in lipase activities and peroxisomal β-oxidation are upregulated. qRT-PCR of selected lipid genes and lipid analyses during the life span of zoospores confirmed these observations. These results highlight...Continue Reading

References

Jun 1, 2002·Science·Susana R NevesRavi Iyengar
Mar 6, 2004·Molecular Plant-microbe Interactions : MPMI·Shuji TaniHoward S Judelson
Nov 4, 2006·Cellular Microbiology·Adrienne R Hardham
Dec 13, 2006·Molecular Plant-microbe Interactions : MPMI·Harold J G Meijer, Francine Govers
Jun 26, 2007·Oecologia·Amanda W J DemopoulosCraig R Smith
May 4, 2010·Nucleic Acids Research·Zhou DuZhen Su
Oct 30, 2012·Bioinformatics·Alexander DobinThomas R Gingeras
May 6, 2014·Frontiers in Microbiology·Maiko KagamiGaku Takimoto
Feb 10, 2016·Frontiers in Cell and Developmental Biology·Ronald J A WandersSacha Ferdinandusse
Apr 5, 2016·Applied Microbiology and Biotechnology·Inga Marie AasenTrond E Ellingsen
Feb 24, 2017·Tropical Life Sciences Research·Hasmidah Md IsaJohan Ismail
Jan 9, 2018·Biochemical and Biophysical Research Communications·Zhongrui Duan, Motoki Tominaga
Jun 22, 2018·Annual Review of Biochemistry·William I Weis, Brian K Kobilka
Jul 4, 2018·Environmental Microbiology·Younès DelleroAlberto Amato
Sep 10, 2019·Progress in Lipid Research·Christian MorabitoFabrice Rébeillé

❮ Previous
Next ❯

Citations

Nov 17, 2020·Frontiers in Microbiology·Hsiu-Chin LinHsin-Yang Chang
Jan 3, 2021·Journal of Bioscience and Bioengineering·Kenshi WatanabeTsunehiro Aki

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.