The Zscan4-Tet2 Transcription Nexus Regulates Metabolic Rewiring and Enhances Proteostasis to Promote Reprogramming.

Cell Reports
Zhou-Li ChengDan Ye

Abstract

Evolutionarily conserved SCAN (named after SRE-ZBP, CTfin51, AW-1, and Number 18 cDNA)-domain-containing zinc finger transcription factors (ZSCAN) have been found in both mouse and human genomes. Zscan4 is transiently expressed during zygotic genome activation (ZGA) in preimplantation embryos and induced pluripotent stem cell (iPSC) reprogramming. However, little is known about the mechanism of Zscan4 underlying these processes of cell fate control. Here, we show that Zscan4f, a representative of ZSCAN proteins, is able to recruit Tet2 through its SCAN domain. The Zscan4f-Tet2 interaction promotes DNA demethylation and regulates the expression of target genes, particularly those encoding glycolytic enzymes and proteasome subunits. Zscan4f regulates metabolic rewiring, enhances proteasome function, and ultimately promotes iPSC generation. These results identify Zscan4f as an important partner of Tet2 in regulating target genes and promoting iPSC generation and suggest a possible and common mechanism shared by SCAN family transcription factors to recruit ten-eleven translocation (TET) DNA dioxygenases to regulate diverse cellular processes, including reprogramming.

References

Sep 22, 1995·The Journal of Biological Chemistry·A J WilliamsT Collins
Jul 29, 2004·Proteins·Kiyean NamChristoph Schumacher
Sep 6, 2005·Gene·Leonard C Edelstein, Tucker Collins
May 5, 2006·Proceedings of the National Academy of Sciences of the United States of America·Malin HernebringThomas Nyström
Sep 5, 2006·Journal of Molecular Biology·Francis C PetersonBrian F Volkman
Jun 8, 2007·Developmental Biology·Geppino FalcoMinoru S H Ko
Jun 27, 2008·Genes & Development·Bin ZhaoKun-Liang Guan
Sep 19, 2008·Genome Biology·Yong ZhangX Shirley Liu
Sep 1, 2009·Cell Stem Cell·Yoshinori YoshidaShinya Yamanaka
Mar 26, 2010·Nature·Michal ZalzmanMinoru S H Ko
Nov 30, 2010·Cell Stem Cell·Saiyong ZhuSheng Ding
May 6, 2011·Bioinformatics·Timothy L Bailey
Aug 26, 2011·Journal of Virology·Ryan O Emerson, James H Thomas
Nov 9, 2011·Cell Research·Athanasia D PanopoulosJuan Carlos Izpisua Belmonte
Jun 23, 2012·Nature·Todd S MacfarlanSamuel L Pfaff
Jun 7, 2013·Nature Communications·Tomokazu AmanoMinoru S H Ko
Oct 25, 2013·Nature·Rahul M Kohli, Yi Zhang
Jun 28, 2016·Cell Stem Cell·Miguel FidalgoJianlong Wang
Aug 1, 2016·Current Topics in Developmental Biology·M S H Ko
Mar 28, 2017·Stem Cells and Development·Soon-Jung ParkMan Ryul Lee
May 6, 2017·Cell Stem Cell·Laura García-PratPura Muñoz-Cánoves
Dec 12, 2018·Cell Stem Cell·Jose Luis SardinaThomas Graf

❮ Previous
Next ❯

Citations

Feb 5, 2021·Frontiers in Cell and Developmental Biology·Vera Garcia-OuteiralDiana Guallar
May 8, 2021·Epigenetics : Official Journal of the DNA Methylation Society·Maud De DieuleveultRachel Deplus

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiovascular Disease & TET2

Cardiovascular diseases are the number one cause of deaths globally. Tet methylcytosine dioxygenase 2 (TET2)-mediated hematopoiesis has been implicated in accelerating heart failure. Here is the latest research on cardiovascular diseases and TET2.