Theaflavin-3-gallate specifically interacts with phosphatidylcholine, forming a precipitate resistant against the detergent action of bile salt

Bioscience, Biotechnology, and Biochemistry
Asako Narai-KanayamaTsutomu Nakayama

Abstract

Black tea is a highly popular beverage, and its pigments, polymerized catechins such as theaflavins (TFs), are attracting attention due to their beneficial health effects. In this study, to test the inhibitory activities of TFs on the intestinal absorption of cholesterol, we investigated their effects on phosphatidylcholine (PC) vesicles in the absence or presence of a bile salt. (-)-Epicatechin gallate, (-)-epigallocatechin gallate, and TFs formed insoluble complexes with PC vesicles. Galloylated TFs such as TF2A, TF2B, and TF3 precipitated far more than other polyphenols. The subsequent addition of taurocholate redispersed the polyphenol-PC complexes, except that a large amount of TF2A remained insoluble. After incubation with taurocholate-PC micelles, TF2A elevated the turbidity of the micelle solution, providing red sediments. The TF2A-specific effect was dependent on the PC concentration. These results suggest that TF2A interacts with PC and aggregates in a specific manner different from catechins and other TFs.

References

Jan 1, 1986·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·D AlvaroM Angelico
Dec 20, 1999·Journal of Agricultural and Food Chemistry·N SubramanianV P Sinkar
Sep 4, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·T P MulderJ M van Amelsvoort
Dec 18, 2001·Journal of Agricultural and Food Chemistry·T TanakaI Kouno
Feb 6, 2002·Bioscience, Biotechnology, and Biochemistry·K KajiyaT Nakayama
Jan 1, 2003·Bioscience, Biotechnology, and Biochemistry·Katsuko KajiyaTsutomu Nakayama
Oct 2, 2003·The Journal of Nutrition·Michael J DaviesShirley C Chen
Jan 4, 2007·Journal of Agricultural and Food Chemistry·Toshiro MatsuiKiyoshi Matsumoto
Aug 28, 2007·Journal of Lipid Research·Chih-Li LinJen-Kun Lin
Oct 31, 2007·Journal of Agricultural and Food Chemistry·Yoshinori UekusaTsutomu Nakayama
Dec 4, 2008·Journal of Agricultural and Food Chemistry·Mario A VermeerHenri O F Molhuizen
Jul 1, 2011·The American Journal of Clinical Nutrition·Xin-Xin ZhengXiao-Hong Huang
Oct 27, 2011·Journal of the American Dietetic Association·Amie KimOlivia J Phung
Feb 28, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Ching-Wei NjauwShih-Huang Tung
Mar 19, 2014·Journal of Agricultural and Food Chemistry·Makoto KobayashiIkuo Ikeda
Jun 29, 2014·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Yimin ZhaoDuo Li
Oct 3, 2014·Bioscience, Biotechnology, and Biochemistry·Toyomi YamazakiTatsuo Watanabe
Oct 17, 2014·Journal of Clinical Biochemistry and Nutrition·Kanako MatsuuraTsutomu Nakayama
Dec 15, 2015·Journal of Agricultural and Food Chemistry·Kazuki OgawaEmiko Yanase
May 5, 2016·The Journal of Nutritional Biochemistry·Akiko SaitoNaomi Osakabe
Oct 11, 2016·Journal of Agricultural and Food Chemistry·Annewieke J W VerloopHarry Gruppen
Oct 21, 2017·Journal of Food Science and Technology·Shicheng LeiXiaoxiong Zeng

❮ Previous
Next ❯

Software Mentioned

Chromato
PRO
StatPlus®

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.