PMID: 2499888Jun 1, 1989Paper

Theileria annulata sporozoite surface antigen expressed in Escherichia coli elicits neutralizing antibody

Proceedings of the National Academy of Sciences of the United States of America
S WilliamsonR Hall

Abstract

Theileria annulata is an economically important protozoan parasite that threatens an estimated 250 million cattle with the disease tropical theileriosis. Development of a defined subunit vaccine is one means of trying to develop control measures against the disease. To this end we have characterized a surface antigen complex of the infective stage (sporozoite), by using a monoclonal antibody that neutralizes sporozoite infectivity in vitro. We have cloned the gene coding for this complex and have demonstrated that a fusion protein expressed from a fragment of this gene elicits strong neutralizing antibodies. Furthermore we provide data on the structure and expression of this gene. In particular we show that the region of the gene, expressed in one clone, codes for a protein segment relatively rich in proline residues. Also we demonstrate that expression of this gene appears to be stage specific, transcripts being present only in the sporoblast and sporozoite stages. The relevance of these findings to the production of a defined subunit vaccine is discussed.

References

Oct 25, 1979·Nature·T W PearsonD A Stagg
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R A YoungR W Davis
May 1, 1986·Veterinary Parasitology·B ShielsC G Brown
Aug 8, 1985·Nature·P C Doherty, R Nussenzweig
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R A Young, R W Davis
Jun 1, 1983·International Journal for Parasitology·A R Walker, S B McKellar
Jan 1, 1980·Journal of Immunological Methods·T W PearsonR S Hwett
Feb 1, 1982·Tropical Animal Health and Production·P M Robinson

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Citations

Nov 1, 1992·Veterinary Immunology and Immunopathology·M KachaniR L Spooner
Jan 15, 1990·Molecular and Biochemical Parasitology·R HallA Tait
Apr 1, 1990·Molecular and Biochemical Parasitology·J GlascodineB Shiels
Jul 1, 1992·Molecular and Biochemical Parasitology·R HallA Tait
Aug 1, 1992·Molecular and Biochemical Parasitology·H A BaylisR Hall
Mar 1, 1992·Molecular and Biochemical Parasitology·V NeneA J Musoke
Jan 1, 1993·Molecular and Biochemical Parasitology·J Dickson, B R Shiels
Aug 1, 1994·Veterinary Parasitology·M W Lightowlers
Nov 1, 1996·The British Veterinary Journal·A J MusokeD McKeever
Mar 25, 2000·International Journal for Parasitology·V NeneA Irvin
May 1, 1997·International Journal for Parasitology·M K Shaw
Apr 26, 2000·Molecular and Biochemical Parasitology·R A SkiltonS P Morzaria
Feb 6, 2007·Parasitology·W I Morrison, D J McKeever
Sep 1, 1994·Parasite Immunology·J D CampbellR L Spooner
Jun 16, 1992·Annals of the New York Academy of Sciences·J L Hodgson
Feb 8, 2014·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·C Rajendran, D D Ray
Jan 13, 2000·Experimental Parasitology·D Prickett, R Hall
Jun 1, 1993·The Veterinary Quarterly·G UilenbergH T Koch
Jun 2, 2007·Molecular and Biochemical Parasitology·William WeirAndrew Tait
Aug 8, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Thillaiampalam SivakumarNaoaki Yokoyama
Sep 21, 2016·Parasite Immunology·V Nene, W I Morrison
Mar 17, 2020·Transboundary and Emerging Diseases·Abubakar A SaaidAbdel Rahim M El Hussein
Aug 15, 2015·PLoS Neglected Tropical Diseases·Marta PieszkoBrian Shiels

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