Theoretical principles of in vitro selection using combinatorial nucleic acid libraries

Current Protocols in Nucleic Acid Chemistry
B Vant-HullD Zichi

Abstract

A new paradigm for drug discovery and biological research has developed from technologies that integrate combinatorial chemistry with rounds of selection and amplification, a technique called in vitro selection or systematic evolution of ligands by exponential enrichment (SELEX). This overview unit discusses nucleic acid libraries that can be used, affinity probability distributions, an equilibrium model for SELEX, and optimal conditions including concentrations and signal-to-noise ratios.

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Citations

Mar 22, 2012·Database : the Journal of Biological Databases and Curation·Jose Cruz-ToledoMichel Dumontier
Nov 30, 2012·TheScientificWorldJournal·Seda OzbalZahide Cavdar
Nov 15, 2012·Journal of Nucleic Acids·Maureen McKeague, Maria C Derosa
Dec 1, 2017·International Journal of Molecular Sciences·Andrew B KinghornJulian A Tanner
Jun 6, 2013·Nucleic Acids Research·Abdullah OzerDavid Shalloway
Jun 1, 2014·Bulletin of Mathematical Biology·Yeon-Jung SeoHoward A Levine
Jun 26, 2014·Current Protocols in Nucleic Acid Chemistry·Vitor B PinheiroPhilipp Holliger
Apr 12, 2021·Talanta·Chen LyuZhouping Wang

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