Theranostic Niosomes for Efficient siRNA/MicroRNA Delivery and Activatable Near-Infrared Fluorescent Tracking of Stem Cells

ACS Applied Materials & Interfaces
Chuanxu YangJørgen Kjems

Abstract

RNA interference-mediated gene regulation in stem cells offers great potential in regenerative medicine. In this study, we developed a theranostic platform for efficient delivery of small RNAs [small interfering RNA (siRNA)/microRNA (miRNA)] to human mesenchymal stem cells (hMSCs) to promote differentiation, and meanwhile, to specifically label the transfected cells for the in vivo tracking purpose. We encapsulated indocyanine green (ICG) in a nonionic surfactant vesicle, termed "niosome", that is mainly composed of a nonionic surfactant sorbitan monooleate (Span 80) and a cationic lipid 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP). This novel ICG-containing niosome system (iSPN) demonstrated highly efficient siRNA and miRNA delivery in hMSCs. Specific inhibition of miR-138, a negative regulator of osteoblast differentiation, was achieved by iSPN/miR-138, which significantly promoted osteogenesis of hMSCs. Furthermore, iSPN exhibited OFF/ON activatable fluorescence upon cellular internalization, resulting in efficient near-infrared labeling and the capability to dynamically monitor stem cells in mice. In addition, iSPN/siRNA achieved simultaneous long-term cell tracking and in vivo gene silencing after implantation in mice....Continue Reading

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Citations

Dec 4, 2019·Current Stem Cell Research & Therapy·Fiona FernandesIndrani Talukdar
Feb 28, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zhiman BaiWei Huang
Sep 10, 2019·Expert Opinion on Drug Delivery·Rita Muzzalupo, Elisabetta Mazzotta
Nov 7, 2019·Chemistry and Physics of Lipids·Marcel ScheidelerRuth Prassl
Jun 17, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Feng DingChuanxu Yang
Jun 24, 2021·Journal of Microencapsulation·Heidi Mohamed Abdel-MageedSaleh Ahmed Mohamed
Nov 16, 2019·ACS Applied Materials & Interfaces·Anna PipernoMaria Teresa Sciortino
Oct 31, 2021·Immunologic Research·Yogesh SharmaReena V Saini

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