Therapeutic angiogenesis induced by human hepatocyte growth factor gene in rat and rabbit hindlimb ischemia models: preclinical study for treatment of peripheral arterial disease

Gene Therapy
Yoshiaki TaniyamaT Ogihara

Abstract

Hepatocyte growth factor (HGF) exclusively stimulates the growth of endothelial cells without replication of vascular smooth muscle cells, and acts as a survival factor against endothelial cell death. Recently, a novel therapeutic strategy for ischemic diseases using angiogenic growth factors to expedite and/or augment collateral artery development has been proposed. We have previously reported that intra-arterial administration of recombinant HGF induced angiogenesis in a rabbit hindlimb ischemia model. In this study, we examined the feasibility of gene therapy using HGF to treat peripheral arterial disease rather than recombinant therapy, due to its disadvantages. Initially, we examined the transfection of 'naked' human HGF plasmid into a rat hindlimb ischemia model. Intramuscular injection of human HGF plasmid resulted in a significant increase in blood flow as assessed by laser Doppler imaging, accompanied by the detection of human HGF protein. A significant increase in capillary density was found in rats transfected with human HGF as compared with control vector, in a dose-dependent manner (P < 0.01). Importantly, at 5 weeks after transfection, the degree of angiogenesis induced by transfection of HGF plasmid was significa...Continue Reading

References

Jan 1, 1992·International Orthopaedics·M Eneroth, B M Persson
Apr 1, 1995·Journal of Vascular Surgery·I DawsonJ Hajo van Bockel
Feb 1, 1993·The British Journal of Surgery·M R Tyrrell, J H Wolfe
Mar 27, 1996·Biochemical and Biophysical Research Communications·S HayashiT Ogihara
Jan 1, 1995·Annual Review of Cell and Developmental Biology·W Risau, I Flamme
Apr 1, 1996·Journal of Biochemistry·K Matsumoto, T Nakamura
Nov 21, 1997·Biochemical and Biophysical Research Communications·K Matsumoto, T Nakamura
Jun 17, 1998·The Journal of Clinical Investigation·T MuroharaJ M Isner

❮ Previous
Next ❯

Citations

Aug 15, 2012·Journal of Artificial Organs : the Official Journal of the Japanese Society for Artificial Organs·Shingo NakamuraTadaaki Maehara
Dec 17, 2002·Clinica Chimica Acta; International Journal of Clinical Chemistry·Hiroshi Funakoshi, Toshikazu Nakamura
Mar 2, 2002·Journal of Diabetes and Its Complications·Hiroyuki Takano, Issei Komuro
Feb 13, 2003·Artificial Organs·Tetsuya IshikawaTakashi Kitajima
Apr 9, 2011·Antioxidants & Redox Signaling·Yukihiro SaitoTadahiro Sasajima
Oct 30, 2013·Human Gene Therapy·Stephen M KaminskyRonald G Crystal
Dec 6, 2008·Stem Cells and Development·Shigejiro IwashimaSeiichi Matsuo
Apr 27, 2012·Experimental Diabetes Research·Roberto BassiPaolo Fiorina
Dec 25, 2002·Circulation Journal : Official Journal of the Japanese Circulation Society·Ryuichi Morishita
Nov 12, 2002·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Ryuichi Morishita
Mar 10, 2004·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Hirofumi MakinoRyuichi Morishita
Oct 30, 2012·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Majid Khazaei, Ensieh Salehi
Dec 1, 2012·Stem Cells Translational Medicine·Yuji HaraguchiTeruo Okano
Dec 3, 2013·BioMed Research International·Munehisa ShimamuraRyuichi Morishita
Dec 5, 2003·Critical Reviews in Clinical Laboratory Sciences·Joel E BarbatoEdith Tzeng
Sep 6, 2014·Expert Review of Cardiovascular Therapy·Hiroyuki SudaRyuichi Morishita
Apr 29, 2014·Expert Opinion on Biological Therapy·Munehisa ShimamuraRyuichi Morishita
Dec 3, 2014·Journal of Molecular and Cellular Cardiology·James RichardsMichael V Autieri
Oct 6, 2011·Advanced Drug Delivery Reviews·Hyun-Ji ParkSeung-Woo Cho
Dec 8, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Chien-Wen ChangSung Wan Kim
Jun 26, 2007·The Annals of Thoracic Surgery·Haruhiko KondohHikaru Matsuda
Jan 24, 2007·Biomaterials·Takashi KitajimaYoshihiro Ito
Sep 15, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Yuuki ImaiKunio Takaoka
Nov 8, 2012·International Journal of Urology : Official Journal of the Japanese Urological Association·Naoki YoshimuraYasuhito Funahashi
Nov 10, 2011·The British Journal of Surgery·N A MughalS Homer-Vanniasinkam

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.